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| 急进高原与平原大鼠肠道双歧杆菌的分子生物学实验对比研究 | |
| 引用本文： | 单体栋,邓芝云,张方信,邵珂,段云,张盼,陈嘉屿,汪泳.急进高原与平原大鼠肠道双歧杆菌的分子生物学实验对比研究[J].中国微生态学杂志,2011,23(3):197-200. |
| 作者姓名： | 单体栋 邓芝云 张方信 邵珂 段云 张盼 陈嘉屿 汪泳 |
| 作者单位： | 1. 兰州大学第二临床医学院,消化科,甘肃,兰州,730000 2. 兰州军区总医院,检验科,甘肃,兰州,730050 3. 兰州军区总医院,消化科,甘肃,兰州,730050 |
| 基金项目： | 全军“十一五”课题项目(06M089); 军区医药卫生科研基金项目(CWS10JA17) |
| 摘 要： | 目的探讨应用荧光定量PCR法对急进高原大鼠肠道双歧杆菌数量变化的分子生物学实验研究。方法 Wistar大鼠60只,随机分为2组:平原对照组和3 848米的高原缺氧组各30只。急进海拔3 848米造成大鼠急性缺氧模型并分别于24、6、d取材,每次随机各取10只,观察肠道菌群中双歧杆菌的变化。结果高原缺氧组双歧杆菌数量明显少于平原对照组(P<0.05)。结论急进高原缺氧复杂环境下,可使肠道有益菌———双歧杆菌的数量减少,可能影响肠道微生态平衡。 |
| 关 键 词： | 急进高原 双歧杆菌 肠道微生态 16S rDNA 荧光PCR |
Comparative study of molecular biology on acute high altitude and plain intestinal Bifidobacterium in rats |
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| SHAN Ti-dong,DENG Zhi-yun,ZHANG Fang-xin,SHAO Ke,DUAN Yun,ZHANG Pan,CHEN Ja-yu,WANG Yong.Comparative study of molecular biology on acute high altitude and plain intestinal Bifidobacterium in rats[J].Chinese Journal of Microecology,2011,23(3):197-200. | |
| Authors: | SHAN Ti-dong DENG Zhi-yun ZHANG Fang-xin SHAO Ke DUAN Yun ZHANG Pan CHEN Ja-yu WANG Yong |
| Institution: | SHAN Ti-dong1,DENG Zhi-yun2,ZHANG Fang-xin3,SHAO Ke3,DUAN Yun3,ZHANG Pan3,CHEN Ja-yu3,WANG Yong3 (1.Department of Gastroenterology,the Second Hospital of Lanzhou University,Lanzhou 730000,China,2.Department of Clinical Laboratory,the General Hospital of Lanzhou Military Area Command,Lanzhou 730050,3.Department of Gastroenterology,China) |
| Abstract: | Objective To observe the change of intestinal Bifidobacterium in rats exposed to acute high altitude by using RT-PCR. Method 60 Wistar rats were randomly divided into plain control group and altitude 3 848 meters group,30 rats in each group.All rats except those in control group were posed acute hypoxia model at radical altitudes of 3 848 meters respectively.Each group was used on Day 2,4 and 6,in order to observe the change of the intestinal Bifidobacterium. Result After exposed to high attitude of hypoxia... |
| Keywords: | Acute high altitude Bifidobacterium Intestinal microecology 16S rDNA Rea1 time PCR |
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