| 基于16S rRNA基因序列分析1例健康人龈上菌群 |
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| 引用本文： | 张松梅,田菲,黄庆丰,赵琰芳,郭晓奎,张富强.基于16S rRNA基因序列分析1例健康人龈上菌群[J].中国微生态学杂志,2011,23(1):44-48. |
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| 作者姓名： | 张松梅 田菲 黄庆丰 赵琰芳 郭晓奎 张富强 |
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| 作者单位： | 1. 上海交通大学医学院附属第九人民医院,口腔修复科,上海市口腔医学重点实验室,上海,200011
2. 上海交通大学医学院,病原生物学教研室,上海,200025 |
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| 基金项目： | 上海市科学技术委员会资助(074119513); 上海市重点(特色)学科建设项目资助(T0202) |
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| 摘 要： | 目的构建细菌16S rRNA基因文库分析健康人龈上菌群的组成。方法取1例健康成年女性龈上菌斑并构建细菌16S rRNA基因文库,分析其龈上菌群组成。结果 1例健康人龈上菌斑细菌的种水平分类有62种,其中可以培养的细菌有34种而尚无法培养的细菌有28种(45.1%);新发现的细菌物种有17种(27.4%);缓症链球菌是克隆子数最多的优势菌种;链球菌属、奈氏菌属和嗜血杆菌属占文库的60%,为主要菌群;构建的细菌16S rRNA基因文库的覆盖率为95%,文库的均匀度值为0.016。结论 1例健康人龈上菌群中45.1%的细菌尚无法分离培养、27.4%的物种尚不清楚,未培养菌及尚不清楚的菌种中可能藏匿着与口腔疾病密切相关的致病菌,全面地了解健康人龈上菌群的组成有助于研究龋病的发病机制。
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| 关 键 词： | 龈上菌斑 微生态 16S rRNA基因 测序 |
The composition of supergingival plaque of one healthy subject based on analysis of 16S rRNA sequence |
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| ZHANG Song-mei,TIAN Fei,HUANG Qing-feng,ZHAO Yan-fang,GUO Xiao-kui,ZHANG Fu-qiang.The composition of supergingival plaque of one healthy subject based on analysis of 16S rRNA sequence[J].Chinese Journal of Microecology,2011,23(1):44-48. |
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| Authors: | ZHANG Song-mei TIAN Fei HUANG Qing-feng ZHAO Yan-fang GUO Xiao-kui ZHANG Fu-qiang |
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| Institution: | 1(1.Department of Prosthodontics,School of Stomatology,the Affiliated Ninth People′s Hospital of Shanghai Jiaotong University,School of Medicine,Shanghai 200011,China;2.Department of Medical Microbiology and Parasitology,Institutes of Medical Sciences,Shanghai Jiaotong University,School of Medicine,Shanghai 200025,China) |
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| Abstract: | Objective To characterize the supergingival plaque composition of one healthy adult by using culture-independent methods.Method 16S rRNA gene clone libraries were utilized to survey the supergingival plaque composition in one healthy individual.Result 62 species-level operational taxonomic units(OTU) were identified in the oral microflora of the subject.Of the 62 OTUs,34 OTUs belong to culture-defined bacterial species and 28 OTUs were non-cultivatable phylotypes.Over 45.1% of the bacterial flora was repres... |
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| Keywords: | Supergingival plaque Microbiota 16S rRNA gene Sequence |
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