| IL-6基因多态性与小儿全身炎症反应综合征的相关性研究 |
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| 引用本文： | 刘培辉,杨跃萍,邹嵘,曹敏.IL-6基因多态性与小儿全身炎症反应综合征的相关性研究[J].中国微生态学杂志,2011,23(1):24-27. |
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| 作者姓名： | 刘培辉 杨跃萍 邹嵘 曹敏 |
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| 作者单位： | 1. 南方医科大学附属深圳市妇幼保健院儿科,广东,深圳,518028
2. 南方医科大学附属深圳市妇幼保健院急诊科,广东,深圳,518028 |
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| 基金项目： | 深圳市科技计划资助项目(医疗卫生类)(200702078) |
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| 摘 要： | 目的探讨白细胞介素-6（Interleuk in-6,IL-6）基因多态性与小儿全身炎症反应综合征（system ic inflamm atory response syndrom e,SIRS）的关系。方法 30例SIRS患儿为SIRS组,随机挑选30例健康体检的小儿为对照组,采用限制片段长度多态分析聚合酶链反应方法（PCR/RFLP）对2组儿童的IL-6基因的-174位点和-572位点基因进行分析;并应用酶联免疫吸附试验法（ELISA）检测2组儿童的血清IL-6水平,观察基因型对血清IL-6水平的影响。结果 IL-6基因-572位点基因型和等位基因频率在2组间分布差异有统计学意义,SIRS组GG基因型和G等位基因频率均显著高于对照组（P〈0.05）;携带G等位基因个体患SIRS的风险约是C等位基因型个体的6.84倍（OR95%CI：2.62～17.89,P〈0.05）;G等位基因携带者IL-6血清含量显著高于CC基因携带者（P〈0.05）。IL-6基因启动子-174位点在SIRS组与对照组中只发现GG基因型,CG和CC基因型在2组中均未发现;SIRS组IL-6血清水平显著高于对照组（P〈0.05）。结论 IL-6基因-572C/G多态性可能是中国汉族儿童SIRS发病的遗传危险因素之一,血清IL-6水平可能受其基因多态性的影响。而IL-6基因-174G/C多态性与中国汉族儿童SIRS发病可能不具有相关性。
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| 关 键 词： | 全身炎症反应综合征 IL-6 多态性 基因 儿童 |
The association between IL-6 polymorphism and systemic inflammatory response syndrome in children |
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| LIU Pei-hui,YANG Yue-ping,ZOU Rong,CAO Min.The association between IL-6 polymorphism and systemic inflammatory response syndrome in children[J].Chinese Journal of Microecology,2011,23(1):24-27. |
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| Authors: | LIU Pei-hui YANG Yue-ping ZOU Rong CAO Min |
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| Institution: | 1(1.Department of Pediatrics;2.Department of Emergency,Shenzhen Maternity ﹠ Child Healthcare Hospital,Southern Medical University,Shenzhen 518028,China) |
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| Abstract: | Objective To investigate the relationship between the polymorphism of the IL-6 gene and SIRS in children and polymorphism effects on serum IL-6 leve1.Method The polymorphism of IL-6 was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method in patients with SIRS(n=30) and normal controls(n=30).The serum level of IL-6 was measured by enzyme-linked immunosorbent assay(ELISA).Result The frequency of the genotype GG of IL-6(-572) site in the SIRS group was significantly ... |
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| Keywords: | Systemic inflammatory response syndrome Interleukin-6 Polymorphism Gene Children |
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