双歧杆菌的完整肽聚糖对大鼠腹腔巨噬细胞TNF-α表达的影响

目的探索双歧双歧杆菌的完整肽聚糖(WPG)对大鼠腹腔巨噬细胞TNF-α的调控作用。方法以WPG刺激大鼠腹腔巨噬细胞;或以NF-κB的抑制剂NAC和ERK抑制剂PD98059分别预先孵育巨噬细胞,再用WPG刺激巨噬细胞,最后用RT-PCR检测巨噬细胞TNF-αmRNA的表达水平。结果 WPG刺激巨噬细胞后,其TNF-αmRNA的表达水平显著高于对照组(P0.01)。但经NAC和PD98059分别预先孵育巨噬细胞后,其TNF-αmRNA的表达水平均明显低于WPG刺激组(P0.01)。结论双歧双歧杆菌的WPG能上调巨噬细胞TNF-αmRNA的表达,这一过程受NF-κB和ERK的调控。

Regulation of whole peptidoglycan of Bifidobacteria on TNF-α of rat peritoneal macrophages

Objective To explore the regulation of whole peptidoglycan（WPG） of Bifidobacteria bifidum on TNF-α of rat peritoneal macrophages.Method Rat peritoneal macrophages were stimulated by WPG.The macrophages were pretreated with NF-κB inhibitor NAC and ERK inhibitor PD98059 respectively,then stimulated by WPG.The expression of TNF-α mRNA of the macrophages was detected by using RT-PCR.Result After the macrophages were stimulated by WPG,its expression level of TNF-α mRNA was obviously higher when compared with control group（P 0.01）.After the macrophages were pretreated with NAC and PD98059 respectively,its expression level of TNF-α mRNA was markedly lower when compared with WPG stimulating group（P 0.01）.Conclusion The WPG of Bifidobacteria bifidum can upregulate the expression of TNF-α mRNA of macrophages through NF-κB and ERK.