| 大肠埃希菌热不稳定肠毒素B亚单位在婴儿双歧杆菌中的表达 |
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| 引用本文： | 罗耀玲,王勇,黄雪萍,冉丹霞,马永平,宋方洲.大肠埃希菌热不稳定肠毒素B亚单位在婴儿双歧杆菌中的表达[J].中国微生态学杂志,2008,20(2):113-115. |
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| 作者姓名： | 罗耀玲 王勇 黄雪萍 冉丹霞 马永平 宋方洲 |
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| 作者单位： | 重庆医科大学,基础医学院生物化学与分子生物学教研室,重庆,400016 |
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| 摘 要： | 目的将大肠埃希菌热不稳定肠毒素B亚单位（LTB）克隆到表达载体pBV220，使LTB基因在大肠埃希菌和双歧杆菌中稳定表达。方法将LTB基因克隆到表达载体pBV220中，再分别转化大肠埃希菌DH5a和婴儿双歧杆菌，使之表达，表达产物用SDS—PAGE鉴定。并通过家兔肠袢实验验证表达蛋白的安全性。结果LTB基因在大肠埃希菌和双歧杆菌中均可稳定表达，毒性实验证明LTB保留轻微的毒性。结论稳定表达LTB的双歧杆菌为今后的口服疫苗佐剂奠定了基础。
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| 关 键 词： | 大肠埃希菌 双歧杆菌 PBV220 LTB |
| 文章编号： | 1005-376X(2008)02-0113-03 |
| 修稿时间： | 2007年10月23 |
Expression of B-subunit of the Escherichia coli heat-labile enterotoxin in Bifidobacterium infantis |
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| LUO Yao-ling,WANG Yong,HUANG Xue-ping,RAN Dan-xia,MA Yong-ping,SONG Fang-zhou.Expression of B-subunit of the Escherichia coli heat-labile enterotoxin in Bifidobacterium infantis[J].Chinese Journal of Microecology,2008,20(2):113-115. |
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| Authors: | LUO Yao-ling WANG Yong HUANG Xue-ping RAN Dan-xia MA Yong-ping SONG Fang-zhou |
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| Institution: | (Department of Biochemistry and Molecular Biology, College of Basic Medical Science, Chongqing Medical University, Chongqing 400016, China) |
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| Abstract: | Objective To stably express B-subunit of the Escherichia coli heat-labile enterotoxin （LTB） in E. coli and Bifidobacterium infantis. Methods LTB gene was cloned into a expression vector pBV220. This vector was designated pBV220-LTB and transformed into B. infantis and E. coli DH5α. The product was determined by SDS-PAGE. Results The ltb gene could stably express in E. coli and B. infantis. The LTB had a gentle toxicity of inducing intestinal juice secretory in rabbit by ansa intestinalis test. Conclusion This study paved the way for using Bifidobacterium-LTB recombinant as an oral vaccine adjuvant in future. |
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| Keywords: | E coli Bifidobacterium pBV220 LTB |
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