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Nisin对幽门螺杆菌生物学作用的实验研究
引用本文:柯连安,胡国平,叶嗣颖.Nisin对幽门螺杆菌生物学作用的实验研究[J].中国微生态学杂志,2003,15(2):96-97,104.
作者姓名:柯连安  胡国平  叶嗣颖
作者单位:1. 华中科技大学,湖北,武汉,430000
2. 湖北孝感职业技术学院医学分院,孝感,432000
摘    要:目的:探讨乳链菌肽(Nisin)在柠檬酸的协同作用下对幽门螺杆菌(Helicobacter pylori,Hp)的生物学作用,寻求一种新的防治Hp微生态制剂,为临床治疗Hp提供理论和实践指导。方法:运用国际通用的药敏试验方法纸片法(Kirby-Bauer)和倾注培养法(Pour Culture)对96例从胃病患者分离出的临床株Nisin和柠檬酸协同作用的生物学实验,然后电镜观察被Nisin作用后的Hp菌株细胞结构并进行分析处理。结果:Nisin在柠檬酸的协同作用下对Hp具有明显的抑杀作用,电镜观察被作用后的Hp菌株细胞质膜破碎和细胞发生球形样变。结论:Nisin作用机制主要表现在对Hp菌株的细胞质膜上。

关 键 词:幽门螺杆菌  乳链菌肽  Nisin  柠檬酸  协同作用  生物学作用  胃疾病
文章编号:1005-376X(2003)02-0096-03

EXPERIMENTAL STUDY ON BIOLOGICAL ACTION OF NISIN AGAINST HELICOBACTER PYLORI
KE Lian an,HU Guo ping,TE Si ying.EXPERIMENTAL STUDY ON BIOLOGICAL ACTION OF NISIN AGAINST HELICOBACTER PYLORI[J].Chinese Journal of Microecology,2003,15(2):96-97,104.
Authors:KE Lian an  HU Guo ping  TE Si ying
Abstract:Objective:To investigate the biological action of a new plypeptide Nisin on Helicobacter pylori and explore the mechanism further Methods:using the international standard methods Kirby Bauer and pour culture to detect the biological action and sensitivity of Nisin to Helicobacter pylori Furthermore to explore the active mechanism by observeing the structure of the cell wall with eletron microscope Results: Nisin can inhibit the growth of 90 Helicobacter pylori strains among 96 strains with effective percentage of 93 75%,and almost all HP strains cell wall structure being attacked by Nisin are raptured or changed Conclusion:Nisin can inhibit the Helicobacter pylori effectively combined with the NM201 ,Electron microscope showed the damaged site on membrane of HP cell ,which lead to the bacterium died
Keywords:Nisin  Helicobacter pylori  Biological action
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