| 耐碳青霉烯类肺炎克雷伯菌的耐药机制研究 |
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| 引用本文： | 李桂秋,崔兰英,陈淑兰,郭大文,赵金英,路娟.耐碳青霉烯类肺炎克雷伯菌的耐药机制研究[J].中国微生态学杂志,2014(11):1272-1274. |
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| 作者姓名： | 李桂秋 崔兰英 陈淑兰 郭大文 赵金英 路娟 |
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| 作者单位： | 哈尔滨医科大学第一附属医院检验科 |
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| 基金项目： | 黑龙江省青年基金(QC2010071) |
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| 摘 要： | 目的了解碳青霉烯类耐药肺炎克雷伯菌及耐药机制。方法对2012-2013年临床分离的耐碳青霉烯类肺炎克雷伯菌共计12株进行分析,药敏采用MIC方法检测,用WHONET 5.6软件进行分析,KPC表型检测采用改良Hodge试验,基因检测采用PCR方法。结果 12株碳青霉烯类耐药肺炎克雷伯菌改良Hodge试验阴性,基因测序为KPC-2型。结论 KPC-2基因是引起本院肺炎克雷伯菌耐药的主要原因。
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| 关 键 词： | 肺炎克雷伯菌 耐药 碳青霉烯酶 |
The mechanisms of resistance of carbapenem-resistant Klebsiella pneumoniae |
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| LI Gui-qiu;CUI Lan-ying;CHEN Shu-lan;GUO Da-wen;ZHAO Jin-ying;LU Juan.The mechanisms of resistance of carbapenem-resistant Klebsiella pneumoniae[J].Chinese Journal of Microecology,2014(11):1272-1274. |
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| Authors: | LI Gui-qiu;CUI Lan-ying;CHEN Shu-lan;GUO Da-wen;ZHAO Jin-ying;LU Juan |
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| Institution: | LI Gui-qiu;CUI Lan-ying;CHEN Shu-lan;GUO Da-wen;ZHAO Jin-ying;LU Juan;Department of Clinical Laboratory,the First Affliated Hospitalof Harbin Medical University; |
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| Abstract: | Objective To understand the mechanisms of carbapenems resistance and drug resistance of Klebsiella pneumoniae. Methods A total of 12 clinical strains of carbapenems-resistant Klebsiella pneumoniae from 2012 to2013 were analyzed,and the MIC method was used for the drug susceptibility test. WHONET 5. 6 software was used for the analysis. KPC phenotypic detection was performed by using modified Hodge test,and genetic test by using PCR. Results The 12 carbapenems-resistant strains was negative to modified Hodge test. Gene sequencing showed that their KPC type were type 2. Conclusion KPC-2 is the main reason causing the drug resistance of Klebsiella pneumoniae in the hospital. |
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| Keywords: | Klebsiella pneumoniae Drug resistance Carbapenemase |
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