| β-葡聚糖对小鼠单核巨噬细胞系RAW264.7的免疫刺激作用 |
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| 引用本文： | 俞晓丽,郭兰芳,陈璐,闻平.β-葡聚糖对小鼠单核巨噬细胞系RAW264.7的免疫刺激作用[J].中国微生态学杂志,2009,21(10):902-903. |
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| 作者姓名： | 俞晓丽 郭兰芳 陈璐 闻平 |
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| 作者单位： | 镇江市第一人民医院,检验科,江苏,镇江,212002 |
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| 摘 要： | 目的研究β-葡聚糖的应用对Balb/c小鼠巨噬细胞株RAW264.7的刺激作用。方法将不同浓度（0～150μg/ml）的β-葡聚糖与Balb/c小鼠来源的巨噬细胞株RAW264.7作用1～7d后,以四甲基偶氮唑蓝（MTT）法检测细胞的增殖情况并绘制细胞生长曲线。结果β-葡聚糖在50～75μg/ml的浓度范围内能够明显地刺激细胞发生增殖。结论适当剂量的β-葡聚糖作用足够时间,RAW264.7细胞系可以发生显著的生长促进效应。
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| 关 键 词： | β-葡聚糖 巨噬细胞 免疫刺激 |
Immunostimulatory effect of beta-1,3-D-glucan on murine macrophage cell line RAW264.7 and its significance |
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| YU Xiao-li,GUO Lan-fang,CHEN Lu,WEN Ping.Immunostimulatory effect of beta-1,3-D-glucan on murine macrophage cell line RAW264.7 and its significance[J].Chinese Journal of Microecology,2009,21(10):902-903. |
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| Authors: | YU Xiao-li GUO Lan-fang CHEN Lu WEN Ping |
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| Institution: | ( Department of Labartory Medcine, the First People＇s Hospital of Zhenjiang, Zhenjiang 212002,China) |
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| Abstract: | Objective To study the immunostimulatory effect of β-1,3-D-glucan on murine macrophage cell line RAW264.7 and its significance. Method Fungal β-glucan particles were resolved in sodium hydroxide and added to the cells at the concentrations indicated below (0 to 150 μg/ml). After incubation for 5 days, MTT assay was performed and the growth curve was completed. Result p-glucan at moderate concentrations 50 to 75 (μg/ml caused apparent proliferation of the murine macrophage cell line RAW264.7. Conclusion With an appropriate dose of (3-1,3-D-glucan and enough disposal time,the growth of RAW264.7 could be promoted significantly and tend to increase. |
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| Keywords: | Macrophage Immunostimulatory |
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