| 球形幽门螺杆菌分子生物学研究 |
| |
| 引用本文： | 佘菲菲,苏东辉,陈月秀,朱苹.球形幽门螺杆菌分子生物学研究[J].中国微生态学杂志,1999,11(1):3-6. |
| |
| 作者姓名： | 佘菲菲 苏东辉 陈月秀 朱苹 |
| |
| 作者单位： | 福建医科大学微生物学教研室,350004 |
| |
| 摘 要： | 为研究幽门螺杆菌（ＨＰ）球形变异本质，作者通过延期培养和采用亚抑菌浓度抗生素，使３株ＨＰ发生球形变异，对弯曲形和球形ＨＰ作了ＳＤＳ－ＰＡＧＥ、免疫印迹及４个毒力基因片段ＰＣＲ和ＰＣＲ－ＳＳＣＰ分析。ＳＤＳ－ＰＡＧＥ图谱显示球形ＨＰ分子量在７４×１０４以上的蛋白含量减少，免疫印迹显示球形ＨＰ１２５×１０４蛋白条带反应减弱，而抗生素诱变的球形ＨＰ分子量为１１×１０４和６３×１０４的蛋白条带反应增强。ＰＣＲ及ＰＣＲ－ＳＳＣＰ结果表明球形ＨＰ的ｈｐａＡ，ＶａｃＡ，ＣａｇＡ和ＵｒｅＡ４个毒力基因片段未发生缺失，但在ｈｐａＡ或ＶａｃＡ基因中存在点突变
|
| 关 键 词： | 球形幽门螺杆菌 免疫印迹 PCR |
| 修稿时间： | 1998-06-16 |
STUDY ON THE MOLECULAR BIOLOGY OF COCCOID HELICOBACTER PYLORI |
| |
| She Feifei,Su Donghui,Chen Yuexiu,et al..STUDY ON THE MOLECULAR BIOLOGY OF COCCOID HELICOBACTER PYLORI[J].Chinese Journal of Microecology,1999,11(1):3-6. |
| |
| Authors: | She Feifei Su Donghui Chen Yuexiu |
| |
| Institution: | She Feifei,Su Donghui,Chen Yuexiu,et al. Department of Microbiology,Fujian Medical University,Fuzhou,350004 |
| |
| Abstract: | In order to reveal the essence of coccoid variety of H.pylori, We first gained three strains of H.pylori with coccoid variety by means of prolonged incubation and subinhibitory antibiotics, and then, studied the differences between the variety strain and its prototype through SDS-PAGE, Western-blot, furthermore, analysing the diferences of four virulent genes by PCR and PCR-SSCP respectively. The results showed that, as in variety strains, the protein containing showed in SDS-PAGE with the molecular weight being above 74 KDa decreased, and hybriditional signal in band 125 KDa weaken, meanwhile, strengthen in band 110 KDa and 63 KDa as showed in western-blot. The results of PCR and PCR-SSCP showed that there were no deletions in genes hpaA, VacA, CagA and UreA in variety strain as compared to their prototypes, but, there may exist the point mutation in genes hpaA or VacA. |
| |
| Keywords: | Coccoid H Pylori\ Western-blot\ PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
