| 1株源于人体的双歧杆菌的PCR扩增和鉴定 |
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| 引用本文： | 丁思,李丽秋,胡新俊,梁金花,王广,马淑霞.1株源于人体的双歧杆菌的PCR扩增和鉴定[J].中国微生态学杂志,2010,22(12):1094-1096. |
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| 作者姓名： | 丁思 李丽秋 胡新俊 梁金花 王广 马淑霞 |
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| 作者单位： | 佳木斯大学微生物教研室,黑龙江佳木斯154007 |
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| 摘 要： | 目的从佳木斯大学健康学生体内分离出1株双歧杆菌。方法利用引物设计软件设计双歧杆菌的引物,通过PCR进行扩增,进行序列分析和鉴定。结果通过BLAST序列比对分析,与GenBank中相应基因同源性为99%。结论确定此菌株为青春双歧杆菌。
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| 关 键 词： | 双歧杆菌 PCR 扩增 鉴定 |
The PCR amplification and identification of the colon bacillus from human body |
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| DING Si,LI Li-qiu,HU Xin-jun,LIANG Jin-hua,WANG Guang,MA Shu-xia.The PCR amplification and identification of the colon bacillus from human body[J].Chinese Journal of Microecology,2010,22(12):1094-1096. |
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| Authors: | DING Si LI Li-qiu HU Xin-jun LIANG Jin-hua WANG Guang MA Shu-xia |
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| Institution: | (Department of Microbiology,Jiamusi University,Jiamusi 154007,China) |
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| Abstract: | Objective To separate a strain of colon bacillus from healthy students of Jiamusi University.Method The colon bacillus primers were designed by Primers design software;PCR was used for amplification;Then the sequence analysis and identification of the strain were performed.Result The strain showed 99% homology to the corresponding gene of GenBank by BLAST sequence analysis.Conclusion The strain is Bifidobacterium adolescent. |
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| Keywords: | Bifidobacterium PCR Amplification Identify |
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