| 鲍曼不动杆菌外膜蛋白与耐药性分析 |
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| 引用本文： | 黄艳飞,陈群.鲍曼不动杆菌外膜蛋白与耐药性分析[J].中国微生态学杂志,2004,16(3):144-145. |
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| 作者姓名： | 黄艳飞 陈群 |
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| 作者单位： | 广东医学院,微生物学教研室,广东,湛江,524023 |
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| 摘 要： | 目的:分析35株鲍曼不动杆菌外膜蛋白(OMP)与耐药性的关系.方法:采用超声物理法制备鲍曼不动杆菌外膜蛋白标本,用变性聚丙烯酰胺凝胶电泳(SDSPAGE)检测外膜蛋白.直接荧光法测鲍曼不动杆菌对环丙沙星的吸收和积累.结果:35株鲍曼不动杆菌都有10条主要OMP带,耐药菌株与敏感菌株相比,发现外膜蛋白在约29 Ku条带处消失,而在26 Ku条带处却明显增强.耐药菌株药物积累量不及敏感菌株,经叠氮钠处理后,积累量上升并接近敏感菌株.结论:鲍曼不动杆菌耐药与外膜蛋白的低通透性和主动外运有关.
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| 关 键 词： | 细菌外膜蛋白 不动杆菌 聚丙烯酰胺凝胶电泳 耐药性 |
| 文章编号： | 1005-376X(2004)03-0144-02 |
| 修稿时间： | 2003年10月5日 |
Studies on the resistance and outer membrane proteins of Acinetobacter baumannii |
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| HUANG Yan-fei,CHEN Qun.Studies on the resistance and outer membrane proteins of Acinetobacter baumannii[J].Chinese Journal of Microecology,2004,16(3):144-145. |
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| Authors: | HUANG Yan-fei CHEN Qun |
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| Abstract: | Objective: To analyze the relationship between the resistance and outer membrane proteins of 35 Acinetobacter baumannii islolates.Methods:Outer membrane proteins(OMP) were extracted by ultrasound physical method and analyzed by SDS-PAGE.Accumulation of ciprofloxacin by A.baumannii was determined by direct Fluorescence method.Results:There have 10 mian OMP tapes in 35 isolates.The analysis of outer menbrane proteins showed that a protein of 29 ku disappeared and 26 ku protein enhanced in resistant isolates.The accumulation of ciprofloxacin in resistant isolates decreased.After treatment with NaN 3,the drug uptake increased to the normal level.Conclusion:Active drug efflux and outer membrane protein permeability decreasement were the factors contributing to the resistance of A.baumannii . |
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| Keywords: | Bacterial outer membrane protein Acinetobacter SDS-PAGE Drug resistance |
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