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一种直接用于PCR扩增的山羊瘤胃微生物DNA提取方法的建立
引用本文:许发芝,吴胜国,李吕木,丁小玲.一种直接用于PCR扩增的山羊瘤胃微生物DNA提取方法的建立[J].中国微生态学杂志,2012,24(1):66-68.
作者姓名:许发芝  吴胜国  李吕木  丁小玲
作者单位:1. 安徽农业大学动物科技学院,安徽合肥,230036
2. 安徽安丰堂动物药业有限公司,安徽合肥,230036
基金项目:安徽省教育厅自然科学基金项目(KJ2011A107)
摘    要:目的建立提取高质量的瘤胃微生物DNA的方法,为采用免培养技术研究山羊瘤胃微生物奠定基础。方法采集山羊瘤胃内容物,用SDS高盐法提取微生物总DNA,以通用引物扩增细菌和古细菌的16SrDNA。结果提取到的瘤胃微生物总DNA片段大于23kb,PCR能够扩增出细菌和古细菌的16SrDNA片段。结论用该提取方法得到的山羊瘤胃微生物总DNA能够满足后续实验的需要。

关 键 词:山羊  瘤胃微生物  DNA

Establishment of a method for extracting goat rumen microbial DNA used in direct PCR amplification
XU Fa-zhi , WU Sheng-guo , LI Lv-mu , DING Xiao-ling.Establishment of a method for extracting goat rumen microbial DNA used in direct PCR amplification[J].Chinese Journal of Microecology,2012,24(1):66-68.
Authors:XU Fa-zhi  WU Sheng-guo  LI Lv-mu  DING Xiao-ling
Institution:1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China;2.Anhui Anfengtang Animal Medicine Industry Co.,Ltd,Hefei 230036,China)
Abstract:Objective To establish a method for extraction of high quality DNA,and lay a foundation for the research on goat rumen microorganism with uncultured technology.Method Goat rumen contents were collected and total microbial DNA was extracted with a high salt extraction of SDS.The 16S rDNA of bacteria and archaeal were amplified by the universal primer.Result The total rumen microbial DNA fragments extracted were larger than 23 kb.The fragments of bacterial and archaeal 16S rDNA could be amplified by PCR.Conclusion The rumen microbial total DNA extracted by this method can satisfy the need of the subsequent research.
Keywords:Goat  Rumen microorganisms  DNA
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