|
|||||
|
|
| 肺炎克雷伯杆菌对氟喹诺酮耐药的机制研究 | |
| 引用本文： | 廖景光,李小燕,李敏妍,张耀森,梁艳容,高炎超,崔志新,黎敏.肺炎克雷伯杆菌对氟喹诺酮耐药的机制研究[J].中国微生态学杂志,2012,24(2):145-148. |
| 作者姓名： | 廖景光 李小燕 李敏妍 张耀森 梁艳容 高炎超 崔志新 黎敏 |
| 作者单位： | 1. 广州市番禺区何贤纪念医院,广东广州,511400 2. 广州呼吸疾病研究所,广东广州,510120 3. 广州市番禺中心医院,广东广州,511400 |
| 基金项目： | 广州市番禺区科技计划资助项目(2007-Z-81-1) |
| 摘 要： | 目的研究肺炎克雷伯杆菌对氟喹诺酮类药物（FQNs）的耐药机制。方法筛选临床分离的对环丙沙星耐药的肺炎克雷伯杆菌共10株,采用微量肉汤稀释法检测菌株对5种氟喹诺酮类药物的MIC值;采用PCR方法检测菌株染色体和质粒携带的喹诺酮耐药基因（gyrA基因、parC基因和qnr基因）并测序;质粒接合试验验证qnr基因的转移性。结果 10株肺炎克雷伯杆菌对5种氟喹诺酮类药物均产生耐药性。扩增产物经测序发现10株肺炎克雷伯杆菌染色体的gyrA基因和parC基因均有突变;有2株菌株（K79和K107）携带qnrA基因,这2株菌的接合菌对喹诺酮抗菌药的MIC值上升了5～30倍;未检测到qnrB阳性的菌株。结论 gyrA和parC基因突变是肺炎克雷菌对氟喹诺酮类产生耐药机制的主要原因,质粒上qnrA基因的存在,也是产生喹诺酮耐药的一个重要因素。 |
| 关 键 词： | 肺炎克雷伯杆菌 氟喹诺酮耐药 gyrA基因 parC基因 qnr基因 |
The mechanism of resistance of Klebsiella pneumoniae to Fluoroquinolones |
|
| LIAO Jing-guang , LI Xiao-yan , LI Min-yan , ZHANG Yao-shen , LIANG Yan-rong , GAO Yan-chao , CUI Zhi-xin , LI Min.The mechanism of resistance of Klebsiella pneumoniae to Fluoroquinolones[J].Chinese Journal of Microecology,2012,24(2):145-148. | |
| Authors: | LIAO Jing-guang LI Xiao-yan LI Min-yan ZHANG Yao-shen LIANG Yan-rong GAO Yan-chao CUI Zhi-xin LI Min |
| Institution: | -yan1,ZHANG Yao-shen3,LIANG Yan-rong1,GAO Yan-chao1,CUI Zhi-xin1,LI Min1(1.Hexian Memorial Hospital,Panyu District,Guangzhou 511400,China;2.Guangzhou Institute of Respiratory Disease,Guangzhou 510120,China;3.Guangzhou Panyu Central Hospital,Guangzhou 511400,China) |
| Abstract: | Objective To investigate the mechanisms of the resistance of Klebsiella pneumoniae(K.pneumoniae) to Fluoroquinolones(FQNs).Method 10 FQNs-resistant K.pneumoniae strains were isolated clinically.Minimal inhibitory concentrations(MIC) against the 5 FQNs were determined with the broth microdilution method.FQNs-resistant genes were detected with polymerase chain reaction(PCR) and the products were sequenced.The qnr gene transferability in vitro was verified by conjugation experiments.Result All the strains of K.pneumoniae were resistant to the FQNs.The results of DNA sequencing showed that all strains had gyrA and parC gene mutation.Two strains(K79 and K107) carried qnrA gene.Their MICs of FQNs were increased by 5 to 30 folds.The qnrB gene was not detected.Conclusion Mutation of gyrA and parC gene is the primary mechanism for the resistance of K.pneumoniae to FQNs.The qnrA gene that plasmid carried is an important factor for FQNs resistance. |
| Keywords: | Klebsiella pneumoniae Fluoroquinolones resistance gyrA gene parC gene qnrA gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |