| 灭活的双歧杆菌对肠上皮细胞粘附及其影响因素的研究 |
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| 引用本文： | 伦永志,黄敏,袁杰利,康白.灭活的双歧杆菌对肠上皮细胞粘附及其影响因素的研究[J].中国微生态学杂志,2000,12(6):314-316. |
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| 作者姓名： | 伦永志 黄敏 袁杰利 康白 |
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| 作者单位： | 1. 大连大学医学院,微生物学教研室,辽宁大连,116027
2. 大连医科大学,微生态学研究所,辽宁大连,116027 |
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| 摘 要： | 目的 观察灭活的青春双歧杆菌对人大肠癌细胞系ＣＣＬ－２２９的粘附以及影响粘附的因素。方法 通过与双歧杆菌活菌比较,灭活的双歧杆菌同样能粘附于肠上皮细胞,并且耗尽培养上清有利于双歧杆菌粘附。结果 粘附具有显著的浓度效应;粘附效果与孵育环境的ｐＨ值有关;高温处理耗尽培养上清对粘附无明显影响。结论 灭活的双歧杆菌可能具有与活菌相同的生态效应。
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| 关 键 词： | 双歧杆菌 肠上皮细胞 粘附 灭活菌 |
| 修稿时间： | 2000-07-10 |
STUDIES ON THE ADHESION AND FACTORS INVOLVED OF HEAT-KILLED BIFIDOBACTERIUM STRAIN TO HUMAN INTESTINAL EPITHELIAL CELL |
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| LUN Yong-zhi,HUANG Min,YUAN Jie-li,KANG Bai.STUDIES ON THE ADHESION AND FACTORS INVOLVED OF HEAT-KILLED BIFIDOBACTERIUM STRAIN TO HUMAN INTESTINAL EPITHELIAL CELL[J].Chinese Journal of Microecology,2000,12(6):314-316. |
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| Authors: | LUN Yong-zhi HUANG Min YUAN Jie-li KANG Bai |
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| Abstract: | The assay investigated the adhesion and factors involved adhesion of heat-killed Bifidobacterium adolescentis strain to human colorectal carcinoma cell line CCL-229.In comparison with live Bifidobacterium strain,heat-killed Bifidobacterium was adhered to human intestinal epithelial cell,and spent culture supernatant comtributed to the adherence of this strain.The results suggestud that the adhesion dependent concentration of Bifidobacterium strain and environment pH that high temperature treated spent culture supernatant couldn't affected signifficantly adhesion.The studies showed:Heat-killed Bifidobacterium strain will be the same ecological effect as live Bifidobacterium strain. |
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| Keywords: | bifidobacterium adhesion intestinal epithelial cell L |
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