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培养条件对筛选根瘤菌突变体的影响
引用本文:陈今朝,张红,顾素芳,韩善华.培养条件对筛选根瘤菌突变体的影响[J].中国微生态学杂志,1999,11(6):346-348.
作者姓名:陈今朝  张红  顾素芳  韩善华
作者单位:1. 涪陵师范专科学校生科系,重庆涪陵,408003
2. 四川师范大学细胞生物学研究室,成都,610068
基金项目:四川省教委重点基金资肋项目
摘    要:将紫外线照射后的宁南2 号根瘤菌(Rhizobium legum inosarum Linglan 2)在不同光照和温度培养下表明,光照强度越大,照射时间越长,根瘤菌损伤就越大,存活率就越低,生长速度就越慢,而且光照还会降低突变株的耐酸碱特性。虽然培养温度对根瘤菌存活率和耐酸耐碱特性影响不大,但根瘤菌的生长和分裂速度则与温度有关。由此说明,培养条件在根瘤菌突变体的筛选中具有重要作用

关 键 词:根瘤菌  突变体  培养条件  影响
修稿时间:1999-05-26

EFFECT OF CULTURE CONDITIONS ON SCREEN OF R.LEGUMINOSARUM MUTANTS
Chen Jinzhao et al. Fuling Teacher's College,Fuling.EFFECT OF CULTURE CONDITIONS ON SCREEN OF R.LEGUMINOSARUM MUTANTS[J].Chinese Journal of Microecology,1999,11(6):346-348.
Authors:Chen Jinzhao Fuling Teacher's College  Fuling
Institution:Chen Jinzhao et al. Fuling Teacher's College,Fuling 408003
Abstract:After ultraviolet irradiation R.Leguminosarum Linglan 2 is cultured in various irradiations and temperature, Study results show that companying irradiation intesity and time increase the injure degree of R. leguminosarum also increases, but its survival percent, growth rate and specific property of acid-alkaili tolerant decrease. Although effects of culture temperature are not very obvious on the specific property of R. leguminosarum in acid-alkaili tolerant, it is relation with the rate of R.leguminosarum growth and division. So, culture conditions are very important in the screen of R. leguminosarum mutants.
Keywords:R  leguminosarum\ Mutant\ Culture condition\ Effect
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