Caspase-3在肝癌组织中的表达及其与HBV感染的关系

目的探讨天冬氨酸特异性半胱氨酸蛋白酶-3（Cysteinyl aspartate-specific proteinase-3，Caspase-3）和生存素（survivin）在原发性肝细胞肝癌（HCC）组织中的表达及其与乙肝病毒（HBV）感染的关系。方法用免疫组化SP法检测21例肝癌组织和10例癌旁组织Caspase-3和survivin的表达．并分析两者表达与HBV感染的关系。结果在肝癌和癌旁组织Caspase-3表达的阳性率分别为33．33％和66．67％（P〈0．05）．survivin在肝癌和癌旁组织表达的阳性率分别为85．71％和0（P〈0．01）。Caspase-3在HBsAg阳性和阴性组表达的阳性率分别为27．78％和66．67％（P〈0．05）；survivin在HBsAg阳性和阴性表达的阳性率分别为88．89％和100％（P〉0．05）。Caspase-3在高、中、低分化组表达的阳性率分别为66．67％、31．25％、33．33％．高分化组与中、低分化组比较差异有显著性（P〈O．05）；survivin在高、中、低分化组表达的阳性率分别为50％、87．5％、100％，高分化组与中、低分化组比较差异有显著性（P〈0．05）。Caspase-3和survivin在HCC的表达呈显著负相关。结论HBV感染能使肝癌组织Caspase-3的表达降低，与survivin共同抑制肝细胞的凋亡，三者在HCC的发生及发展过程中起一定作用。

The expression significance of caspase-3 in the tissne of liver cancer and the relationship with HBV infection

Objective To investigate the effect of HBV infection to the caspase-3(Cysteinyl aspartate-specific proteinase-3) and survivin expression in primary liver cancer.Method Immunohistochemistry was used to detect the expression of caspase-3 and survivin in 21 cases cancer tissue and 10 cases cancer surrounding tissue.Results In 21 cases cancer tissue and 10 cases cancer surrounding tissue,the positive expression ratio of caspase-3 were(33.33%) and(70.00%),respectively(P<0.05);the positive expression ratio of survivin were 85.71% and 0,respectively(P<0.01).In HBsAg positive and negative gruop,the positive expression ratio of caspase-3 were(27.78%) and 100%,respectively(P<0.05).In high and low differentiation gruop.the positive expression ratio of caspase-3 were 66.67% and 32.29%,respectively(P<0.05);the positive expression ratio of survivin were(50.00%) and 93.75%,respectively(P>0.05).The expression of caspase-3 and survivin in primary liver cancer was negatively correlated.Conclusion HBV could suppress the expression of caspase-3 in primary liver cancer,and this maybe the possible mechanism of primary liver cancer formation.