乳酸杆菌发酵滤液对人宫颈癌细胞株Hela细胞的细胞周期和细胞凋亡的影响

目的观察乳酸杆菌DM9811发酵滤液对宫颈癌细胞株Hela细胞的细胞周期和细胞凋亡的影响,探索乳酸杆菌发酵滤液对宫颈癌细胞作用的可能机制。方法用光镜、电镜和流式细胞仪分析不同浓度乳酸杆菌DM9811发酵滤液对Hela细胞凋亡的诱导效果;用流式细胞仪分析不同浓度乳酸杆菌DM9811发酵滤液对Hela细胞细胞周期的影响。结果（1）乳酸杆菌DM9811发酵滤液可诱导宫颈癌Hela细胞凋亡。形态学观察处理后的Hela细胞,可见细胞变形,细胞皱缩,体积变小,细胞间隙增大,细胞核固缩。流式细胞仪分析,1%、2%的乳酸杆菌DM9811发酵滤液在48、72h可诱导Hela细胞凋亡;5%的乳酸杆菌发酵滤液在24、48和72h均可诱导Hela细胞凋亡。（2）乳酸杆菌DM9811发酵滤液阻滞宫颈癌Hela细胞于S期,不同浓度的乳酸杆菌发酵滤液作用24、48和72h均可使S期细胞比阴性对照组增多。结论乳酸杆菌DM9811发酵滤液可诱导部分Hela细胞凋亡,其对Hela细胞的生长抑制作用可能通过S期阻滞实现。

The effects of Lactobacillus fermentation filtrate on the cell cycle and apoptosis of human cervical carcinoma Hela cells in vitro

Objective To explore the effect of Lactobacillus fermentation filtrate and its components on the cell cycle and apoptosis of Hela cell as well as its possible mechanism. Method Optical microscope, electron microscope and FCM were used to analyse the apoptosis of Hela cell treated with different concentration of DM9811 fermentation filtrates. FCM was used to analyse the cell cycle of the differently treated Hela cells. Result The fermented filtrate of Lactobacillus could induce Hela apoptosis. Morphological observation showed cell deformation, cell shrinkage to smaller size, cell gap increase and nuclear pyknosis. Flow cytometry analysis showed that the 1 % and 2% of lactic acid bacteria fermentation filtrates induced the apoptosis of Hela cells at 48 h and 72 h, while the 5% of the filtrate induced the apoptosis at 24 h, 48 h and 72 h, respectively. The fermented filtrate of Lactobacillus could reduce the number of the cells in G1 phase and increase that in S phase. Conclusion The fermented filtrate of Lactobacillus could induce a part of the Hela cells to apoptosize. Its growth inhibition effect on Hela cells may contribute to S-phase arrest.