| 海藻糖对双歧杆菌DM8504菌株冷冻干燥保护效果的研究 |
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| 引用本文： | 温朗聪,苗蔚荣,等.海藻糖对双歧杆菌DM8504菌株冷冻干燥保护效果的研究[J].中国微生态学杂志,1997,9(2):37-39. |
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| 作者姓名： | 温朗聪 苗蔚荣 |
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| 作者单位： | 大连医科大学科达药业微生态研究所!大连116024(温朗聪，袁杰利，李兵，康白)，大连理工大学化工学院!大连116011(苗蔚荣，刘传斌) |
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| 摘 要： | 本文就海藻糖对ＤＭ８５０４双歧杆菌的冻干保护效果进行了研究，考察了海藻糖浓度、保护剂溶液与菌泥混合时间对ＤＭ８５０４冻干存活率的影响，结果证实该保护剂对ＤＭ８５０４双歧杆菌有良好的冻干保护效果，优于其它保护剂。
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| 关 键 词： | 海藻糖 双歧杆菌 冷冻干燥 |
THE EFFECTS OF TREHALOSE ON BIFIDOBACTERIUM DM8504 DURING LYOPHILIZATION AND STORAGE |
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| Wen Langcong,et al..THE EFFECTS OF TREHALOSE ON BIFIDOBACTERIUM DM8504 DURING LYOPHILIZATION AND STORAGE[J].Chinese Journal of Microecology,1997,9(2):37-39. |
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| Authors: | Wen Langcong |
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| Institution: | Wen Langcong,et al. Institute of Microecology,Dalian Keda Pharmaceutical Co. Ltd. Dalian Medical University,Dalian 116023 |
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| Abstract: | In this paper, trehalose was used as a cryoprotectant for DM8504 bifidobacterium. The effects of trehalose concentration and mixing time of trehalose solution with centrifuged Bifidobacterium mud on the survival rate of Bifidobacterium during lyophilization and the long-storage were studied. We found that 20% trehalose solution and 20-40 min. mixing time before freezing was essential to get a high survival rate for Bifidobacterium during freeze-drying. DM8504 bifidobacterium powder with trehalose was far more stable than other. |
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| Keywords: | Trehalose Bifidobacterium Lyophilization |
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