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| 壳寡糖对肝癌细胞SMMC-7721中Bcl-2和Caspase-3表达的影响 | |
| 引用本文： | 罗福文,万莹,张翠丽,姜妙娜,贾玉杰.壳寡糖对肝癌细胞SMMC-7721中Bcl-2和Caspase-3表达的影响[J].中国微生态学杂志,2009,21(10):906-909. |
| 作者姓名： | 罗福文 万莹 张翠丽 姜妙娜 贾玉杰 |
| 作者单位： | 1. 大连医科大学附属一院,普外科,辽宁,大连,116011 2. 大连医科大学生物技术专业,辽宁,大连,116044 3. 大连医科大学,病理生理学教研室,辽宁,大连,116044 |
| 摘 要： | 目的检测壳寡糖对人肝癌SMMC-7721细胞的抑制效果及对凋亡调控蛋白Bcl-2和Caspase-3的影响。方法采用噻唑蓝（MTT）法检测不同浓度壳寡糖对肝癌细胞SMMC-7721细胞增殖的抑制作用,并利用荧光Hoechst33258染色法检测细胞凋亡状况。最后通过免疫细胞化学方法研究壳寡糖对肝癌细胞SMMC-7721中Bcl-2和Caspase-3表达的影响。结果壳寡糖能够抑制SMMC-7721细胞增殖,并且促进SMMC-7721细胞的凋亡,并且壳寡糖能够上调促凋亡蛋白Caspase-3的表达和降低抑制凋亡蛋白Bcl-2的表达。结论壳寡糖对人肝癌SMMC-7721细胞的增殖有抑制作用,此作用可能是通过促进Caspase-3的表达和抑制Bcl-2的表达来实现的。 |
| 关 键 词： | 壳寡糖 肝癌 SMMC-7721细胞 免疫细胞化学 Caspase-3 Bcl-2 |
Effect of oligochitosan on the expression of Bcl-2 and Caspase-3 in SMMC-7721 cells of hepatocarcinoma |
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| LUO Fu-wen,WAN Ying,ZHANG Cui-li,JIANG Miao-na,JIA Yu-jie.Effect of oligochitosan on the expression of Bcl-2 and Caspase-3 in SMMC-7721 cells of hepatocarcinoma[J].Chinese Journal of Microecology,2009,21(10):906-909. | |
| Authors: | LUO Fu-wen WAN Ying ZHANG Cui-li JIANG Miao-na JIA Yu-jie |
| Institution: | LUO Fu-wen , WAN Ying, ZHANG Cui-li , JIANG Miao-na, JIA Yu-jie ( 1. Department of General Surgery,the First Affiliated Hospital of Dalian Medical University, Dalian 116011, China ;2. Department of Pathophysiology,Dalian Medical University, Dalian 116044, China; 3. Biotechnology Major, Dalian Medical University, Dalian 116044, China) |
| Abstract: | Objective To investigate the inhibitory function of oligochitosan on human hepatocelular carcinoma cell line SMMC-7721 and determine the effect of oligochitosan on the expression of Caspase-3 and Bcl-2 in the hepatoma cell line. Method MIT assay was applied for evaluation of proliferation of SMMC-7721 treated by different concentrations of oligochitosan. Hoechst 33258 coloration was adopted to detect cell apoptosis. Immunocytochemistry was utilized to measure the expression of Caspase-3 and Bcl-2 in the SMMC-7721 cells treated by oligochitosan. Result Oligochitosan inhibited the proliferation of SMMC-7721 cells and accelerated cell apoptosis. The expression of Caspase-3 and Bcl-2 were respectively higher and lower in oligochitosan treated group than those in the control group. Conclusion The mechanisms of oligochitosan inhibiting the proliferation of SMMC-7721 cells were possibly that oligochitosan up-regulates the expression of Caspase-3 and down-regulates the expression of Bcl-2. |
| Keywords: | Caspase-3 Bcl-2 Oligochitosan Hepatoma SMMC-7721 cell Immunocytochemistry Caspase-3 Bcl-2 |
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