复合微生态制剂对幼刺参营养成分和 酶活力的影响

目的研究复合微生态制剂对幼刺参体壁营养成分、几种消化酶和免疫酶活力的影响。方法在封闭式循环系统中投喂不同的微生态制剂进行30 d刺参养殖实验。结果投喂液态复合微生态制剂组和粉状复合微生态制剂组的刺参体壁的粗脂肪、总糖、粗蛋白含量最高,两组与对照组相比差异有统计学意义(Ps0.05)。添加微生态制剂的3个实验组刺参肠道蛋白酶、淀粉酶活力均比对照组高,且差异有统计学意义(Ps0.05)。实验组刺参的肠道、体壁、体液过氧化氢酶活力均比对照组高,其中投喂液态复合微生态制剂实验组活力最高。实验组刺参组织的酸性磷酸酶和碱性磷酸酶活力也明显高于对照组(与对照组相比差异有统计学意义,Ps0.05)。结论微生态制剂可以有效改善幼刺参体壁营养成分,促进机体消化活力,提高刺参免疫力。

Effects of compound probiotics on nutrition components and enzyme activity in juvenile sea cucumber Apostichopus japonicus

Abstract: Objective To study the effects of compound probiotics onthe nutrient composition and activities of several digestive and immune enzymes in juvenile Apostichopus japonicus. Methods Juvenile Apostichopus japonicas were fed with different probiotics in a closed recirculating aquaculture system for 30 days. Results The contents of fat, total sugar and protein in the body walls of Apostichopus japonicus in liquid compound probiotics group and powdered compound probiotics group were the highest, significantly different from those in the control group respectively(Ps<0.05). The activities of protease and amylase in Apostichopus japonicus''s intestine in the three experiment groups were higher significantly than those in control group respectively(Ps<0.05). The activity of catalase in the intestine, body wall and coelomic fluid of Apostichopus japonicas in the three experiment groups were higher than that in the control group, with that in liquid compound probiotics group being the highest. The activities of acid phosphatase and alkaline phosphatase in Apostichopus japonicus''s intestine, body wall and coelomic fluid in experiment groups were higher significantly than those in the control group respectively(Ps<0.05). Conclusion Probiotics can effectively improve the nutrient composition in body wall, promote the digestive activity and the immunity of Apostichopus japonicus.