| 牙鲆肠道乳酸菌的分离和鉴定 |
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| 引用本文： | 陈营,王福强,蒲红宇,杜强.牙鲆肠道乳酸菌的分离和鉴定[J].中国微生态学杂志,2002,14(2):70-72. |
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| 作者姓名： | 陈营 王福强 蒲红宇 杜强 |
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| 作者单位： | 1. 烟台大学化学生物理工学院,山东,烟台,264005
2. 大连水产学院,养殖系,辽宁,大连,116023 |
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| 摘 要： | 目的：根据微生态学原理，从健康牙鲆的肠道固有菌群中分离乳酸菌。方法：需氧与厌氧培养法。结果：两种方法获得了不同的结果，用LBS（pH6.5）直接分离，从19株分离菌中只获得6株乳酸菌，其中1株P15为乳杆菌；而用LBS（pH5.4）和SL(pH5.4）先富集后分离得到了许多单一的乳杆菌菌落。对所分离的乳杆菌进行生化鉴定，均符合该菌的生化特征。牛津杯抑菌试验显示，乳酸菌对弧菌均有抑制作用，其中乳杆菌P15在pH6.8和pH7.5生长良好，并对弧菌有强力的抑制作用；而在pH8.0和pH8.5时该菌生长不良且无抑菌活性。
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| 关 键 词： | 牙鲆 乳酸菌 肠道菌群 分离 鉴定 |
| 文章编号： | 1005-376X(2002)02-0070-03 |
| 修稿时间： | 2002年1月30日 |
ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM GASTROINTESTINAL TRACT OF FLOUNDER |
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| CHEN Ying,WANG Fu-qiang,PU Hong-yu,et al..ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM GASTROINTESTINAL TRACT OF FLOUNDER[J].Chinese Journal of Microecology,2002,14(2):70-72. |
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| Authors: | CHEN Ying WANG Fu-qiang PU Hong-yu |
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| Institution: | CHEN Ying,WANG Fu-qiang,PU Hong-yu,et al . |
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| Abstract: | Objective:Based on the microecological principle,lactic acid bacteria were isolated from the autochthonous flora in gastrointestinal tract of healthy flounders.The results were different as the two methods were used.6 strains of lactic acid bacteria were obtained from 19 strains of intestinal bacteria with the LBS plate (pH6.5) directly.All of the bacteria isolated from LBA plate(pH5.4) and SL plate (pH5.4) afterenrichment were pure Lactobacillus sp .and shown to be the characterization of Lactobacillus .In Oxford cup assay,lactic acid bacteria were shown to have inhibition effect on Vibrio .Among the bacteria, Lactobacillus strain P15 has great growth rate and powerful inhibition activity in pH6.8 and pH7.5.But no any inhibition activity in pH8.0 and pH8.5. |
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| Keywords: | Flounder Lactic acid bacteria Intestinal flora |
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