双歧杆菌防治鼠伤寒沙门菌感染的实验研究

目的　以小鼠为模型，研究双歧杆菌在体内对鼠伤寒沙门菌（Ｓａｌｍｏｎｅｌｌａ ｔｙｐｈｉｍｕｒｉｕｍ ，ＳＴＭ） 感染的防治作用。方法　分别用大剂量悉复欢、Ｂ．ｂｉｆｉｄｕｍ 、生理盐水（ＮＳ） 给三组小鼠灌胃，再用ＳＴＭ 攻击，观察小鼠经上述不同处理前后肠道双歧杆菌数量和ＳＴＭ 攻击后粪便ＳＴＭ 培养阳性率，阳性标本ＳＴＭ 分离值及小鼠ＳＴＭ 感染率；同时用双歧杆菌、悉复欢、双歧杆菌加悉复欢分别治疗ＳＴＭ 感染的小鼠，观察并比较疗效。结果　１． 大剂量悉复欢使用可使小鼠肠道内双歧杆菌明显降低，而双歧杆菌灌胃则肠道双歧杆菌明显增多。双歧杆菌灌胃的小鼠粪便ＳＴＭ培养阳性率、阳性粪便ＳＴＭ 值明显低于用大剂量悉复欢和ＮＳ 的小鼠，小鼠ＳＴＭ 感染发病率也明显较低。２． 对于ＳＴＭ 感染鼠，双歧杆菌与悉复欢联合治疗效果最好。结论　１． 双歧杆菌在体内对ＳＴＭ 有拮抗作用；能预防和减少ＳＴＭ 感染发生；２． 在ＳＴＭ 感染时，先用悉复欢，再用双歧杆菌可以达到预期疗效，双歧杆菌对鼠伤寒沙门菌感染有辅助治疗作用。

THE PREVENTIVE AND THERAPEUTIC EFFECTS OF BIFIDOBACTERIUM ON SALMONELLA TYPHIMURIUM INFECTION IN MICE

Objective To investigate whether Bifidobacterium bifidum (B bifidum)have preventive and/or therapeutically effects on STM in fection in mice Methods B bifidum,physical saline (NS) of large dose of Ciprofloxacin was infused into mouse stomachs in three groups separately Then,salmonella typhimurium(STM) was infused in all three groups The feces were cultured for identifying Bifidobacterium and STM individually The Bifidobacterium and STM were counted also Results Bifidobacterium in intestines of the mice infused with large dose of ciprofloxacin decreased But the Bifidobacterium in intestines of the mice infused B bifidum increased The rate of positive STM culture in feces and the morbidity of STM infection in the groups that were infused with B bifidum were lower than the figures in groups infused with large dose of Ciprogloxacin or NS The effect of combination of ciprofloxacin and B bifidum was greater than that of ciprofloxacin or B bifidum alone in STM infection Conclusions Bifidobacterium is antagonistic and inhibitory to STM and has preventive effects on STM infection It is batter to use ciprofloxacin prior to B bifidum in the treatment of STM infection However,B bifidum serves as an auxiliary therapy only