| 乙肝病毒突变核心蛋白基因的克隆及序列分析 |
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| 引用本文： | 张洪勤,李佩珍,应俊.乙肝病毒突变核心蛋白基因的克隆及序列分析[J].中国微生态学杂志,2008,20(6):573-574. |
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| 作者姓名： | 张洪勤 李佩珍 应俊 |
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| 作者单位： | 温州医学院生物实验教学中心,浙江温州,325000 |
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| 摘 要： | 目的克隆发生长片段缺失的乙肝病毒核心蛋白基因（HBV-C），并对其进行DNA序列和蛋白质结构分析。方法通过PCR从1株乙型肝炎病毒中扩增得到发生长片段缺失的HBV-C基因，利用TA克隆将PCR产物克隆人pUCm—T载体并进行测序、同源性比较和蛋白质结构分析。结果PCR扩增出的HBV-C基因经序列分析表明长度为454bp，其核苷酸序列缺失了220—317bp之间的98个碱基，造成从74个氨基酸起发生移码突变。结论成功克隆发生长片段缺失的HBV-C基因，为表达及功能研究奠定了基础。
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| 关 键 词： | 乙肝病毒 核心蛋白基因 缺失 基因克隆 |
Cloning and analysis of the mutational core gene of the hepatitis B virus |
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| ZHANG Hong-qin,LI Pei-zhen,YING Jun.Cloning and analysis of the mutational core gene of the hepatitis B virus[J].Chinese Journal of Microecology,2008,20(6):573-574. |
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| Authors: | ZHANG Hong-qin LI Pei-zhen YING Jun |
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| Institution: | ( Central Laboratory of Biology of Wenzhou Medical College, Wenzhou 325027, China) |
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| Abstract: | Objective To clone and analyze the deleted form of the hepatitis B virus core gene.Methods The deleted form of hepatitis B virus core gene was obtained by PCR from a hepatitis B virus genome.The PCR product was ligated into pUCm-T vector and sequenced.Result The cloned segment was 454 bp with 98 bp between 220 bp and 317 bp being lost.Conclusion The deleted form of hepatitis B virus core gene was cloned successfully which could be used for expression and function researches. |
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| Keywords: | Hepatitis B virus Core gene Deletion Gene cloning |
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