| 一种快速定量检测益生菌制品中植物乳杆菌的方法 |
| |
| 引用本文： | 陈臣,任婧,周方方,刘振民,郭本恒.一种快速定量检测益生菌制品中植物乳杆菌的方法[J].中国微生态学杂志,2013(12):1439-1443,1450. |
| |
| 作者姓名： | 陈臣 任婧 周方方 刘振民 郭本恒 |
| |
| 作者单位： | 光明乳业股份有限公司研究院,乳业生物技术国家重点实验室,上海200436 |
| |
| 基金项目： | 科技部“十二五”国家科技支撑计划课题：发酵乳制品乳酸菌菌种与发酵剂的研究与开发（2013BAD18B01）;科技部“十二五”国家863课题：优良益生菌高效筛选与应用关键技术（2011AA100901）. |
| |
| 摘 要： | 目的建立快速定量检测植物乳杆菌的方法，排除近缘菌的干扰。方法利用SMM系统筛选植物乳杆菌种特异序列，根据特异序列设计引物进行实时荧光定量PCR反应。结果设计的引物具有良好的种特异性，检测限为2．26×10^2CFU／mL。结论该方法快速灵敏，可以在实际生产中应用。
|
| 关 键 词： | 植物乳杆菌 SMM系统 实时荧光定量PCR |
A rapid quantitative detection method of Lactobacillus plantarum in the probiotics products |
| |
| CHEN Chen,REN Qing,ZHOU Fang-fang,LIU Zhen-min,GUO Ben-heng.A rapid quantitative detection method of Lactobacillus plantarum in the probiotics products[J].Chinese Journal of Microecology,2013(12):1439-1443,1450. |
| |
| Authors: | CHEN Chen REN Qing ZHOU Fang-fang LIU Zhen-min GUO Ben-heng |
| |
| Institution: | State Key Laboratory of Dairy Biotechnology, Institute of Bright Dairy ＆ Food Co. , Ltd. , Shanghai 200436 |
| |
| Abstract: | Objective To establish a specific method for the rapid and quantitative detection of Lactobacillus plantarum without any interference. Methods SMM system was used to identify L. plantarum specific sequences. The primers were chosen according the specific sequences to develop a real-time PCR assay. Results The designed primers had good specificity for L. plantarum without interference from L. paraplantarum and L. pentosus and the detection limit of the method was 2.26 ×10^2 CFU/mL. Conclusion The method was rapid and accurate and could be used in actual production. |
| |
| Keywords: | Lactobacillus plantarum SMM system Real time PCR |
| 本文献已被 维普 等数据库收录！ |
