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益气健脾中药颗粒剂对早期断奶仔猪肠道菌群的影响
引用本文:赵娟,贺东昌,武果桃,张李俊,姚敬明,李文刚,薛俊龙,任杰,杨忠,孟冬霞,刘一飞.益气健脾中药颗粒剂对早期断奶仔猪肠道菌群的影响[J].中国微生态学杂志,2013(10):1139-1142.
作者姓名:赵娟  贺东昌  武果桃  张李俊  姚敬明  李文刚  薛俊龙  任杰  杨忠  孟冬霞  刘一飞
作者单位:[1]山西省农科院畜牧兽医研究所,山西太原030032 [2]山西省畜牧遗传育种中心,山西太原030009
基金项目:山西省科技攻关项目(20110311032)
摘    要:目的研究中药复方颗粒剂对断奶仔猪肠道微生态的影响。方法实验选用人工感染大肠埃希菌引起腹泻的28日龄断奶仔猪54头,随机平均分为3组,每组3个重复,Ⅰ组抗生素治疗组、Ⅱ组中药复方颗粒剂治疗组、Ⅲ组不用任何药物治疗的空白对照组。分别于用药后第14天时,每组随机抽取1头屠宰,无菌采集空肠,回肠和盲肠内容物,利用平板计数法测定各肠段大肠埃希菌、双歧杆菌和乳酸杆菌数量。结果 Ⅰ组双歧杆菌、乳酸杆菌及大肠埃希菌的数量均显著小于Ⅲ组(P〈0.05);Ⅱ组双歧杆菌和乳酸杆菌的数量显著大于Ⅲ组(P〈0.05),大肠埃希菌数量显著小于Ⅲ组(P〈0.05); n组双歧杆菌和乳酸杆菌的数量极显著大于Ⅰ组(P〈0.01),Ⅱ组与Ⅰ组大肠埃希菌数量差异无统计学意义(P〉0.05)。结论该中药复方颗粒剂具有显著增加断奶仔猪肠道中双歧杆菌和乳酸杆菌的数量,降低大肠埃希菌数量,减少腹泻频率的作用。

关 键 词:中药  断奶仔猪  大肠埃希菌  双歧杆菌  乳酸杆菌  腹泻

The influence of Chinese herbal medicine compound with Tonifying Qi and spleen on intestinal micro-ecology of weaned piglets
Institution:ZHAO Juan1 , HE Dong-chang1 , WU Guo-tao1,ZHANG Li-jun1,YAO Jing-ming1,LI Wen-gang1 ,XUE Jun-long1, REN Jie1, YANG Zhong2,MENG Dong-xia1, LIU Yi-fen1(1. Institute of Animal Husbandry and Veterinary Science, Shanxi Academy of Agricultural Science,Taiyuan 030032,China ; 2. Animal Genetic Breeding Center in Shanxi Provine,Taiyuan 030009,China )
Abstract:Objective To study the influence of Chinese herbal medicine compound on intestinal micro-ecology of weaned piglets. Methods Fifty-four weaned piglets which were artificially infected by Escherichia coli at 25 days old were equally divided into threegroups with three replicates, and reared in isolated pens. Group 1 and 2 were respectively treated with antibiotics and Chinese herbal med-icine compound, while Groups 3 was blank control. One weaned piglet was randomly selected from each group on pre-administer period 14d after administered, then took contents of jejunum, ileum and cecum and used CFU to measure the amounts of Escherichia coli, Lactoba-cillus and Bifidobacterium on all taken intestines. Results The amounts of Escherichia coli,Bifidobacterium and Lactobacillus in Group1 were reduced significantly compared with the Group 3 ( P 〈 0. 05). The amounts of Bifidobacterium and Lactobacillus in Group 2 wereincreased significantly compared with the Group 3 (P 〈0. 05),while the amounts of Escherichia coli were reduced compared with theGroup 3 (P 〈0. 05) . The amounts of Bifidobacterium and Lactobacillus in Group 2 were increased significantly compared with the Group1 (尸〈0.01),while the amounts of Escherichia coli were not significant (P 〉0. 05). Conclusion This Chinese herbal medicine compound can increase the amounts of Bifidobacterium and Lactobacillus and decrease the amounts of Escherichia coli in weaned piglets intestinal contents significantly. Meanwhile it can decrease diarrhea rate in weaned piglets
Keywords:Chinese herbal  Weaned piglets  Escherichia coli  Bifidobacterium  Lactobacillus  Diarrhea
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