茶多酚对ApoE~(-/-)小鼠肠道菌群多样性的影响

目的以高脂饲料饲养的SPF级C57 BL/6 ApoE-/-小鼠作为动物模型,研究茶多酚采食对其肠道菌群多样性的影响。方法通过随机饮水的方式给予ApoE-/-小鼠0.4、0.8和1.6 g/L的茶多酚,处理14 d时用PCR-DGGE分析对照组(CK)和茶多酚组小鼠新鲜粪便中肠道菌群的相似性和多样性。结果 UPGMA聚类分析表明,低剂量茶多酚组(LTP)、中剂量茶多酚组(MTP)这两组与对照组(CK)、高剂量茶多酚组(HTP)聚为两大簇。PCA分析显示,LTP组与CK组、MTP组、HTP组分别聚集在不同位置,有明显界限;多样性数据分析显示:CK组DGGE图谱的丰富度和Shannon-Wiener指数(H')与茶多酚组差异无统计学意义(P0.05);CK组与MTP组的均匀度(E)差异存在统计学意义(P0.05,P=0.015),说明中剂量茶多酚作用14 d后与对照组肠道菌群的菌群分配相比均一性显著下降。结论连续处理14 d时CK组与LTP组、MTP组小鼠肠道菌群差异有统计学意义,即茶多酚对ApoE-/-小鼠肠道菌群多样性有显著影响。

Effects of tea polyphenols on intestinal microflora diversity in ApoE-/-mice by PCR-DGGE

Objective To study the effects of green tea polyphenols(GTP) on the diversity of intestinal microflora molecular profile in mice.Methods SPF ApoE-/-mice were fed with high-fat diet and drinking water containing 1.6 g/L,0.8 g/L and 0.4g/L GTP respectively for 14 days.Fecal samples were collected during the period of the mice feeding with GTP.Diversity of intestinal microbiota in feces was studied with PCR-DGGE method.Results Similarity cluster analysis of DGGE profile showed that low-dose GTP group and medium-dose GTP group were clustered into two major clusters with control group and high-dose GTP group.Principal component analysis indicated that the low-dose GTP group and control group,medium-dose GTP group and high-dose GTP group were located in different regions respectively.No remarkable differences were observed in the richness,Shannon-Wiener indexes of intestinal microflora between the control and GTP groups by statistical analysis(P>0.05);Significant differences were observed in the evenness degree of intestinal microflora between control group and medium-dose GTP group(P<0.05,P=0.015).Conclusion Tea polyphenols can affect the diversity of mice intestinal microflora significantly.