| 铜绿假单胞菌的MALDI-TOF-MS检测方法的建立 |
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| 引用本文： | 谢田刚,王运铎,郑秋月,赵昕.铜绿假单胞菌的MALDI-TOF-MS检测方法的建立[J].中国微生态学杂志,2012,24(10):938-940. |
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| 作者姓名： | 谢田刚 王运铎 郑秋月 赵昕 |
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| 作者单位： | 1. 大连市中心医院,辽宁大连,116033
2. 辽宁出入境检验检疫局,辽宁大连,116001 |
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| 摘 要： | 目的 建立利用基质辅助激光解吸电离飞行时间质谱仪( MALDI-TOF-MS)对铜绿假单胞菌的快速检测方法.方法 通过MALDI-TOF-MS法对铜绿假单胞菌进行检测分析,并与生化鉴定方法相比较.结果 MALDI-TOF-MS对铜绿假单胞菌的检测后得到肽指纹图片及相关质谱数据,建立MALDI-TOF-MS对铜绿假单胞菌的快速检测方法.结论 MALDI-TOF-MS方法检测铜绿假单胞菌准确快速、操作简单等特点,可发展成为食品检验铜绿假单胞菌的重要(辅助)工具.
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| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱仪 铜绿假单胞菌 鉴定 |
The establishment of MALDI-TOF-MS method for Pseudomonas aeruginosa detection and identification |
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| XIE Tian-gang,WANG Yun-duo,ZHENG Qiu-yue,ZHAO Xin.The establishment of MALDI-TOF-MS method for Pseudomonas aeruginosa detection and identification[J].Chinese Journal of Microecology,2012,24(10):938-940. |
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| Authors: | XIE Tian-gang WANG Yun-duo ZHENG Qiu-yue ZHAO Xin |
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| Institution: | 1.Dalian Municipal Central Hospital,Dalian 116033,China;2,Liaoning Entry-Exit Inspection and Quarantine Bureau,Dalian 116001,China) |
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| Abstract: | Objective To establish a rapid detection and identification method for Pseudomonas aeruginosa by Matrix assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).Methods MALDI-TOF-MS was applied to test the Pseudomonas aeruginosa,and the results were compared to those of biochemical identification.Results Peptide mass fingerprinting(PMF) and mass-spectrum data of Pseudomonas aeruginosa were obtained by MALDI-TOF-MS.A rapid detection and identification method for Pseudomonas aeruginosa by MALDI-TOF-MS was established.Conclusion MALDI-TOF-MS is a precise,rapid and simple method for detection and identification of Pseudomonas aeruginosa,thus can be developed as an important(assistant) tool for detection and identification of Pseudomonas aeruginosa in food inspection. |
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| Keywords: | Matrix assisted laser desorption/ionization time of flight mass spectrometry Pseudomonas aeruginosa Identification |
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