嗜热链球菌高密度发酵过程的优化方案探讨

本研究介绍了嗜热链球菌（Streptococcus thermophilus）高密度发酵过程的优化方案。首先从培养基优化方案入手,讨论了单因素法与正交实验法在培养基优化过程中的利弊。第二,介绍了培养条件以及发酵过程的优化,包括pH、培养温度、搅拌转速、接种量和培养时间。第三,介绍了菌体的后处理过程,包括降温处理、超声解链、离心冻干。

Optimization of high density fermentation process of Streptococcus thermophilus

In this article,we introduced the optimization of high density fermentation process of Streptococcus thermophilus.Starting with the optimization of culture medium,the pros and cons of single factor analysis method and orthogonal testing method were discussed.Then the optimization of culture condition and fermentation process such as the pH,temperature,agitator speed,inoculating quantity,and incubation time were described.Finally,the post-treatment process of bacteria including cooling treatment,ultrasound treatment,as well as centrifugation and lyophilization were introduced.