杭州市萧山区女性HPV感染及分子流行病学研究

目的调查和研究23种HPV基因亚型在杭州市萧山区女性中感染状况以及分子流行病学特点,为预防HPV感染和宫颈癌防治提供依据。方法利用DNA杂交技术检测妇科门诊样本,并进行基因分型,比较不同基因型流行病学特点。结果在15 414例样本中HPV感染者3 230例,感染率为20.95%,其中高危亚型检出2 203例,占感染者的68.20%,低危亚型569例,占17.62%,同时感染高危亚型和低危亚型的458例,占14.18%。单一感染2 295例,占感染者的71.05%,两重感染642例,占19.88%,≥3重的多重感染293例,占9.07%。23个亚型中16型最多,其次为52和58型。≤25年龄段感染比例最高,与其他年龄段感染率比较差异有统计学意义（P〈0.01）。结论高危亚型、单一感染为本地区常见类型,HPV16、52和58为主要感染亚型;HPV感染具有年龄分布特点。

Molecular epidemiological study of Human papillomavirus infection among women patients in Xiaoshan district,Hangzhou City

Objective To study the prevalence and molecular epidemiological characteristics of 23 genotypes of human papillomavirus(HPV) in Xiaoshan district,Hangzhou City,and provide theoretical basis for preventing and treating uterine cervical cancer.Method 15 414 samples were detected for HPV genotypes by using DNA hybridization technique,and the molecular epidemiological characteristics of 23 HPV types were compared.Result 3 230 cases were HPV,with the positive rate of 20.95%.2 203 cases belonged to high-risk type only,accounting for 68.20% of HPV positive cases.569(17.62%) cases were low-risk type,while 458(14.18%) cases were mixed with high-risk type and low-risk type.Subtype HPV16 occupied the first of all high-risk types,followed by subtype HPV52 and HPV58.2 295 cases were single HPV genotype,accounting for 71.05% of HPV positive cases.642(19.88%) cases were double HPV genotypes,and 293(9.07%) cases were multiple types of 3 or more.Compared with other age groups,infection rate of the group with ages ≤25 was the highest,and the difference was significant.Conclusion High-risk,single genotype is the most common HPV infection type,and HPV16,HPV52 and HPV58 are the major subtypes.The distribution of HPV is associated with age.