首页 | 本学科首页   官方微博 | 高级检索  
   检索      

肠道益生菌体外抑菌活性的研究
引用本文:赵佳锐,范小兵,杭晓敏,杨虹.肠道益生菌体外抑菌活性的研究[J].中国微生态学杂志,2006,18(2):88-90,93.
作者姓名:赵佳锐  范小兵  杭晓敏  杨虹
作者单位:1. 上海交通大学生命科学技术学院,上海,200240
2. 上海交大昂立股份有限公司,生物医药研究所,上海,200030
摘    要:以6种常见肠道致病菌作为指示菌,研究了来自健康青年和儿童肠道的25株益生菌发酵浓缩液的体外抑菌效果.进一步研究了抑菌作用良好的LMa1和LMa3经pH调节、热处理、蛋白酶处理以及发酵过程中的抑菌活性变化.短链有机酸作为发酵终产物的主要成分之一,其抑菌效果也被测定.结果表明,抑菌物质包括短链有机酸(主要为乙酸和乳酸)和一类对热稳定、对部分蛋白酶敏感的物质,可能是某种细菌素.低pH是表现抑菌活性的必要条件,但仅有低pH(如低pH缓冲液)并无明显抑菌效果.

关 键 词:益生菌  双歧杆菌  乳杆菌  抑菌  有机酸
文章编号:1005-376X(2006)02-0088-04
收稿时间:2005-04-06
修稿时间:2005-04-06

An in vitro study of antibacterial activity of 25 strains of probiotics from human gastrointestinal tract
ZHAO Jia-rui,FAN Xiao-bing,HANG Xiao-min,YANG Hong.An in vitro study of antibacterial activity of 25 strains of probiotics from human gastrointestinal tract[J].Chinese Journal of Microecology,2006,18(2):88-90,93.
Authors:ZHAO Jia-rui  FAN Xiao-bing  HANG Xiao-min  YANG Hong
Institution:1. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China ;2. Shanghai Jiao Da Onlly Co. , Dd, Shanghai 200030, China
Abstract:Fermentation concentrated fluids of 25 isolations of Bifidobacteria and Lactobacillus from gastrointestinal tracts of healthy youths and children were studied to detect the antibacteriral activities in vitro, using 6 species of normal intestinal pathogenes indicator organisms. LMal and LMa3 showed higher effects on all 6 species. Acetic acid and lactic acid, main component of fermentation concentrated fluids, which showed antibacterial activity, were confirmed as antibacterial substance, but not the only. Antibacterial substance was pH-dependent and heat-stable. Antibacterial activity of substance was lost after treatment with Trypsin and Papain, which indicates that the substance was a bacteriocin.
Keywords:Probiotics  Bifidobacteria  Lactobacillus  Antibacterial activity  Organic acid
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号