健康猪直肠粪便中沙门菌I类整合子与耐药基因的检测

目的了解安徽省规模化猪场健康猪直肠粪便中沙门菌分离株多重耐药情况及其与I类整合子和耐药基因的携带关系。方法采用标准K-B纸片法对22株沙门菌分离株进行15种抗生素敏感试验;应用PCR技术对沙门菌分离株进行I类整合子及耐药基因检测。结果 22株沙门菌分离株中有20株(90.91%)对2种以上抗生素耐药,属于多重耐药株,羧氨苄青霉素-四环素-卡那霉素-氯霉素-氟苯尼考是主要多重耐药谱;22株沙门菌中有19株(86.4%)携带I类整合子,tetB、aph(3)-IIa和cmlA基因分别检出最高。结论沙门菌多重耐药性与整合子携带之间的关系密切,耐药表型测定结果与耐药基因检测结果基本一致,基因组DNA携带的耐药基因种类多于质粒。

Detection of Class I integrons and antibiotic resistance genes of Salmonella isolated from recta faeces of clinically healthy swine

Objective To understand the relationship between multiple-resistance of Salmonella isolates from recta faeces of clinically healthy swine and the conditions of carrying class I integrons and antibotic resistance genes.Method The susceptibility testing of 22 strains of Salmonella isolates to 15 antibiotics were performed by means of K-B disc method.PCR technique was used to detect the presence of class I integrons and antibiotic resistance genes.Result 20 strains of the 22 isolates（90.91%） exhibited resistance to at least two antibiotics.The dominating multiple resistant spectrum was Amoxicillin-Tetracycline-Kanamycin-Chloromycetin-Florfonicol.In the 22 Salmonella strains,19 strains（86.4%） carried class I integrons and the most prevalent genes detected by PCR were tetB,aph（3）-IIa and cmlA genes.Conclusion A close correlation exists between the multiple resistance and the carrier condition of class I integrons.The phenotype of multiple resistance is consistent with the antibiotic resistance genes.The genome DNA carries more genes than plasmid does.