昂立植物乳杆菌对腹腔感染大鼠肠道微生态的影响

目的探讨经肠道补充昂立植物乳杆菌(LP-Onlly)对腹腔感染大鼠肠道微生态的影响.方法大鼠经盲肠结扎穿孔法及颈静脉空肠置管制成腹腔感染模型后,分别给予肠外营养(PN组)和PN 昂立植物乳杆菌(LP-Onlly组)持续5 d,第6天处死,取盲肠内粪便进行肠菌群培养计数及细菌种群DNA指纹图谱分析.结果 LP-Onlly组大鼠肠道内的乳杆菌和双歧杆菌数量较单纯PN组的大鼠为多,而肠道内的潜在致病菌产气荚膜梭菌数量较单纯PN组的大鼠少,DNA指纹图谱也显示LP-Onlly组大鼠优势菌群的基因条带和正常大鼠具有较高的一致性,而PN组则差异有显著性.结论 LP-Onlly能纠正腹腔感染大鼠PN时的肠道菌群紊乱, 调理肠道微生态平衡.

The effect of Lactobacillus plantarum LP-Onlly on the gut microflora of the rats with abdominal infection

The effect of L.plantarum LP-Onlly supplement by gut on the microflora of the SD rats with abdominal infection was studied.After making the model of cecal ligation and perforation,SD rats divided into two groups were given parenteral nutrition(PN group) and PN LP-Onlly (LP-Onlly group) via the needle jejunostomy and neck vein for five days.The feces in cecum were cultured and analyzed by bacterial group DNA fingerprinting spectrum.The results showed that the number of Lactobacillus and Bifidobacillus in LP-Onlly group was lower in the former than that of latter.And DNA fingerprinting figure also shoued that the gene bands of main gut bacterial group in LP-Onlly group similar to that of normal rats,but that of PN group was clearly different to that of normal rats.These results suggested that LP-Onlly as probiotics could correct the intestinal flora disturbance and adjust the gut microflora balance.