| 利用基本培养基初步筛选牛瘤胃中纤维素降解菌 |
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| 引用本文： | 于连林,靳利娥,鲍卫仁.利用基本培养基初步筛选牛瘤胃中纤维素降解菌[J].中国微生态学杂志,2009,21(2):122-124. |
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| 作者姓名： | 于连林 靳利娥 鲍卫仁 |
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| 作者单位： | 1. 太原理工大学,煤科学与技术教育部与山西省重点实验室,山西,太原,030024
2. 太原理工大学,煤科学与技术教育部与山西省重点实验室,山西,太原,030024;太原理工大学,化学工程与技术学院,山西,太原,030024 |
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| 摘 要： | 目的初步筛选牛瘤胃中纤维素降解菌。方法分别采用基本培养基（牛肉膏蛋白胨培养基、马丁培养基），利用好氧、兼性和厌氧3种不同的培养方法进行初选，初步分离牛瘤胃中的细菌与真菌，再通过复选培养基（加入微晶纤维素），筛选降解纤维素的菌种。结果筛选分离出降解纤维素的1株厌氧细菌和1株厌氧真菌。结论此实验方法简单易行，能够有效地从牛瘤胃中筛选出生长良好的纤维素降解菌。
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| 关 键 词： | 瘤胃 纤维素降解菌 筛选分离 |
Preliminary screen of cellulolytic bacteria in rumen by minimum medium |
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| YU Lian-lin,JIN Li-e,BAO Wei-ren.Preliminary screen of cellulolytic bacteria in rumen by minimum medium[J].Chinese Journal of Microecology,2009,21(2):122-124. |
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| Authors: | YU Lian-lin JIN Li-e BAO Wei-ren |
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| Institution: | YU Lian-lin ,JIN Li-e ,BAO Wei-ren ( 1. Key Laboratory for Coal Science and Technology of Shanxi Province and Ministry of Education, Taiyuan University of Technology, Taiyuan 030024, China ;2. Institute of Chemical Engineering and Technology, Taiyuan University of Technology, Taiynan 030024, China) |
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| Abstract: | Objective To preliminarily screen celluloytic bacteria in rumen. Method The bacteria and fungi were isolated by two different culture media;The aerobic,facultative anaerobic and anaerobic microorganisms were isolated by three different culture methods;Then, the cellulolytic bacteria were screened by the reselective culture medium （contai- ning microceUulose）. Result A strain of anaerobic bacteria and a strain of anaerobic fungi were screened and isolated. Conclusion The method used was simple and efficient for screening and isolating celluloytic bacteria in tureen. |
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| Keywords: | Rumen Cellulolytie bacteria Screen and isolate |
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