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GF昆明小鼠主要重量系数及血液生理指标的测定
引用本文:李文霞,曾本华,袁静,唐欢,魏泓.GF昆明小鼠主要重量系数及血液生理指标的测定[J].中国微生态学杂志,2011,23(9):780-783.
作者姓名:李文霞  曾本华  袁静  唐欢  魏泓
作者单位:第三军医大学实验动物学教研室,重庆,400038
基金项目:国家重点基础研究发展计划“973”资助项目
摘    要:目的测定无菌KM小鼠体重、体长、尾长、脏器系数、血常规值。方法选取8只全雄无菌KM小鼠,8只SPF级测量体重、体长、尾长;检测红细胞总数(RBC),血红蛋白(HGB),红细胞压积(HCT),平均红细胞体积(MCV),血小板总数(PLT),白细胞总数(WBC),白细胞分类(DLC),平均血红蛋白含量(MCH),平均血红蛋白浓度(MCHC)等血液生理指标;解剖采集小鼠心脏、肝脏、脾脏、肺、肾、睾丸、盲肠,称重,计算脏器系数(脏器系数=脏器湿重/体重×100)。结果体长、尾长、心脏、脾脏、肺、肾脏、脑、睾丸、及血液生理指标等项目差异无统计学意义(P>0.05),体重、肝脏、盲肠项目差异有统计学意义(P<0.05)。结论所测数值为研究者提供基础数据,建立国内无菌KM小鼠背景资料。

关 键 词:GF动物  脏器系数  血常规

Measured the weight factor of the major organs and blood physiological indicators of GM-KM mouse
LI Wen-xia,ZENG Ben-hua,YUAN Jing,TANG Huan,WEI Hong.Measured the weight factor of the major organs and blood physiological indicators of GM-KM mouse[J].Chinese Journal of Microecology,2011,23(9):780-783.
Authors:LI Wen-xia  ZENG Ben-hua  YUAN Jing  TANG Huan  WEI Hong
Institution:LI Wen-xia,ZENG Ben-hua,YUAN Jing,TANG Huan,WEI Hong(Department of Laboratory Animal Science,Third Military Medical University,Chongqing 400038,China)
Abstract:Objective To determine the GF-KM mice's body weight,body length,tail length,organ coefficient,routine blood test.Method 8 GF-KM mice and 8 SPF-KM mice were used in this study.Body weights,body lengths,tail lengths were measured at first.Then,blood was collected for detection of the total number of red blood cells(RBC),hemoglobin(HGB),hematocrit(HCT),mean corpuscular volume(MCV),the total number of platelets(PLT),total white blood cells(WBC),leukocyte differential count(DLC),the average hemoglobin content(MC...
Keywords:GF mouse  Organ coefficient  Blood routine  
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