| 厌氧菌微量生化板的研制 |
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| 引用本文： | 李建秋,吴晓岩,熊德鑫.厌氧菌微量生化板的研制[J].中国微生态学杂志,2000,12(1):49-50. |
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| 作者姓名： | 李建秋 吴晓岩 熊德鑫 |
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| 作者单位： | 1. 黑龙江省医院,150036
2. 北京解放军304医院,100037 |
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| 摘 要： | 利用预成酶的原理，研制厌氧菌微量化生鉴定板，用国际标准菌株检测其符合率达９５．９％，重复率达９６．３３％，用己知引进参考菌株检测，其符合率达９４．８４％，重复率达９４．３３％，而对临床分离的革兰阴性细菌的符合率达８１％，对临床分离的革兰阳性细菌符合率达９０％，此法效果与国外ＡＰＩ－２０Ａ产品效果类似，但此法具有快速，操作简便，重复性好等优点，值得在广大临床检测中推广和普及。
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| 关 键 词： | 厌氧菌 微量生化板 快速诊断 |
| 文章编号： | 1005-376X(2000)02-0049-02 |
| 修稿时间： | 1999-11-15 |
DEVELOPMENT OF MICROTITER PLATE FOR ANAEROBIC BIOCHEMICAL IDENTIFICATION |
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| Li Jianqiu,Wu Xiaoyan,Xong Dexin.DEVELOPMENT OF MICROTITER PLATE FOR ANAEROBIC BIOCHEMICAL IDENTIFICATION[J].Chinese Journal of Microecology,2000,12(1):49-50. |
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| Authors: | Li Jianqiu Wu Xiaoyan Xong Dexin |
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| Institution: | Li Jianqiu,Wu Xiaoyan,Xong Dexin.
(Hospital of Heilongjiang province,Haerbin 150036) |
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| Abstract: | Using pre-coated enzyme, we developed a microtiter plate anaerobic biochemical identification. With international standard and identified anaerobes, coincidence rates were 95.92% and 94.84%, reproducible rates were 96.33% and 94.33% respectively. Also, coincidence rates were 81% and 90% for elinical isolated gram-negative and gram-positive organisms. Bacterial identification revealed that similar results were observed by using our microtiter plate compared to API20A identifier, while it has many advantages, such as rapid examination,eas to operate, and comparable duplication, etc. We think that it is worth popularizing in hospitals. |
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| Keywords: | amaerpbe micro biochemical plate rapid diagnosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
