| 两种博尔纳病病毒株持续感染PC-12细胞模型的建立 |
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| 引用本文： | 张亮,刘霞,黄荣忠,马丽华,金戈,徐晓艳,邓靖,李文娟,房亮,谢鹏.两种博尔纳病病毒株持续感染PC-12细胞模型的建立[J].中国微生态学杂志,2011,23(12):1064-1067,1073. |
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| 作者姓名： | 张亮 刘霞 黄荣忠 马丽华 金戈 徐晓艳 邓靖 李文娟 房亮 谢鹏 |
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| 作者单位： | 1. 重庆医科大学附属第一医院神经内科,重庆400016;重庆医科大学神经科学研究中心,重庆400016;重庆市神经生物学重点实验室,重庆400016
2. 重庆医科大学神经科学研究中心,重庆400016;重庆市神经生物学重点实验室,重庆400016 |
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| 基金项目： | 国家重大科学研究计划(973计划)项目(2009CB918302); 重庆市卫生局医学科学技术研究项目(2010-2-102); 重庆市自然科学基金计划(CSTC,2010BB5393) |
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| 摘 要： | 目的构建两种博尔纳病病毒株持续感染的PC-12细胞模型,为研究博尔纳病病毒感染致病机制及比较两种病毒株致病特点的差别提供工具。方法将BDV Strain V株和Hu株分别感染PC-12细胞系,并进行传代培养,最后通过Real time FQ RT-PCR、Western blot、间接免疫荧光方法进行病毒核酸和蛋白的检测。结果在培养传代6代后,两种病毒株感染的PC-12细胞均可检测到BDV核酸及蛋白。结论 BDV Strain V株和Hu株均可在PC-12细胞中复制和表达,两种博尔纳病病毒株持续感染PC-12细胞模型构建成功。
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| 关 键 词： | 博尔纳病病毒 病毒株 PC-12 细胞模型 |
Establishment of two PC-12 cell models of persistent infection by two respective types of strains of Borna disease virus |
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| ZHANG Liang
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LIU Xia
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HUANG Rong-zhong
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MA Li-hua
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JIN Ge
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XU Xiao-yan
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DENG Jing
,
LI Wen-juan
,
FANG Liang
,
XIE Peng.Establishment of two PC-12 cell models of persistent infection by two respective types of strains of Borna disease virus[J].Chinese Journal of Microecology,2011,23(12):1064-1067,1073. |
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| Authors: | ZHANG Liang LIU Xia HUANG Rong-zhong MA Li-hua JIN Ge XU Xiao-yan DENG Jing LI Wen-juan FANG Liang XIE Peng |
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| Institution: | ZHANG Liang1,2,3,LIU Xia1,HUANG Rong-zhong1,MA Li-hua2,JIN Ge1,XU Xiao-yan2,DENG Jing1,LI Wen-juan2,FANG Liang2,XIE Peng1,3(1.Department of Neurology,the First Affiliated Hospital of Medical University,Chongqing 400016,China,2.Institute of Neuroscience,Chongqing Medical University,3.Key Laboratory of Neurobiology Designated by the Municipal of Chongqing,China) |
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| Abstract: | Objective Two PC-12 cell models of persistent infection by two types of strains of Borna disease virus(BDV) were respectively established to provide a tool for studying pathogenesis of BDV infection and comparing pathogenesis feature between these two types of BDV strains.Method PC-12 cells were infected respectively with BDV strain Hu and strain V,and subcultivated for passages.BDV nucleic acid and expression of BDV protein were then detected by Real time FQ RT-PCR,Western blot and indirect immunofluoresce... |
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| Keywords: | Borna disease virus Virus strain PC-12 Cell model |
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