| 多重耐药铜绿假单胞菌群体感应系统与主动外排基因表达的研究 |
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| 引用本文： | 李美芬,徐卫东,王莹超,解范迪.多重耐药铜绿假单胞菌群体感应系统与主动外排基因表达的研究[J].中国微生态学杂志,2011,23(12):1115-1118. |
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| 作者姓名： | 李美芬 徐卫东 王莹超 解范迪 |
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| 作者单位： | 1. 南京医科大学附属苏州医院检验科,江苏苏州,215008
2. 上海市江湾医院检验科,上海,200434 |
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| 摘 要： | 目的研究临床多重耐药铜绿假单胞菌群体感应(QS)系统与主动外排泵MexAB-OprM系统基因表达水平与抗生素耐药关系。方法收集苏州市立医院和上海市江湾医院2011年2月至6月间临床标本中分离的铜绿假单胞菌,定量分析细菌生物被膜形成能力;MIC法检测细菌抗生素耐药性,用多重聚合酶链反应(PCR)扩增群体感应系统lasI、lasR及主动外排泵系统mexA基因,实时定量逆转录RT-PCR检测lasI、lasR和mexA基因的相对表达量。结果临床样本分离出84株铜绿假单胞菌,其中产生物被膜菌58株,占比69%;多重耐药菌共24株,占比28.6%;多重耐药菌株中产生物被膜有11株,占45.8%;多重耐药菌中mexA基因表达上调有18株,占75%;lasI基因表达上调有8株,占33.3%。结论多重耐药菌株的生物被膜形成率显著低于非多重耐药组,多重耐药铜绿假单胞菌的主动外排泵MexAB-OprM系统基因表达出现显著上调,生物被膜菌的lasI基因表达显著上调而lasR基因的表达无明显变化。
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| 关 键 词： | 多重耐药铜绿假单胞菌 主动外排基因 抗生素耐药 生物被膜菌 |
Gene expression of quorum sensing system and active efflux gene in clinical multidrug resistant Pseudomonas aeruginosa |
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| LI Mei-fen
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XU Wei-dong
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WANG Ying-chao
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XIE Fan-di.Gene expression of quorum sensing system and active efflux gene in clinical multidrug resistant Pseudomonas aeruginosa[J].Chinese Journal of Microecology,2011,23(12):1115-1118. |
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| Authors: | LI Mei-fen XU Wei-dong WANG Ying-chao XIE Fan-di |
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| Institution: | LI Mei-fen1,XU Wei-dong1,WANG Ying-chao1,XIE Fan-di2(1.Department of Laboratory Medicine,the Affiliated Suzhou Hospital of Nanjing Medical University,Suzhou 215008,China,2.Shanghai Jiangwan Hosipital,Shanghai 200434,China) |
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| Abstract: | Objective To investigate the gene expression of quorum sensing(QS) systems and active efflux pump MexAB-OprM in clinical multidrug resistant Pseudomonas aeruginosa and analyze the association with antibiotic resistance.Method Clinical isolates of Pseudomonas aeruginosa were collected in Suzhou municipal hospital and Shanghai Jiangwan hospital from February to June,2011.The bacterial biofilm forming ability was detected by quantitative assay;bacterial antibiotic resistance was detected by minimum inhibitory ... |
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| Keywords: | Multidrug resistant Pseudomonas aeruginosa Active efflux gene Antibiotic resistance Biofilm bacteria |
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