双歧杆菌脂磷壁酸与5-氟尿嘧啶联用的抗肿瘤研究

目的探讨双歧杆菌脂磷壁酸与5-氟尿嘧啶（5-Fu）联用对H22荷瘤小鼠的抗肿瘤作用及免疫功能的影响。方法双歧杆菌脂磷壁酸单独或联合5-Fu处理H22荷瘤Balb／c小鼠，定期测量肿瘤大小，观察小鼠一般状况；计算抑瘤率、血红细胞数和白细胞数，取脾和胸腺计算脏器指数；HE染色分析肿瘤组织变化；MTT法检测小鼠脾T淋巴细胞增殖转化功能以及ELISA法检测小鼠脾淋巴细胞分泌IFN-γ含量。结果双歧杆菌脂磷壁酸及5-Fu单独应用均可抑制肿瘤生长，但单独5-Fu处理组小鼠一般状况差，毒性反应重；双歧杆菌脂磷壁酸与5-Fu联合应用，与单独5-Fu处理组比较，不仅抑瘤率明显提高（P〈0．01），且荷瘤小鼠一般状况改善，白细胞数升高，脏器指数增加，小鼠脾T淋巴细胞增殖能力强，脾淋巴细胞分泌IFN-γ，水平提高；光镜观察HE染色瘤体组织，双歧杆菌脂磷壁酸处理组可见大量炎症细胞浸润。结论双歧杆菌脂磷壁酸联合5-FU能增强化疗的抑瘤作用，并能扭转化疗引起的免疫低下现象，起到增效减毒作用。

Anti-tumor study of lipoteichoic acid of Bifidobacterium in combination with 5-fuluorouracil

Objective To investigate the anti-tumor and positive immuno-regulation effects of lipoteichoie acid （LTA） of Bifidobacterium on the mice beating inoculated H22 cells treated with 5-FU. Methods Tumor-bearing mice were treat with 5-FU alone, LTA alone or LTA in combination with 5-FU. The general status and tumor size were observed and measured regularly. The growth inhibiting rate （IR） of tumor, spleen index （ SI）, thymus index （ TI）, counts of blood leucocyte and erythrocyte were detected. HE staining was used to analyze the histologic change of tumor. MTT assay was used to evaluate the proliferation of T lymphocytes. ELISA was used to detect the change of IFN-γ. Results Both 5-FU and LTA had inhibition effect on tumor-growth. The general status of mice treated with 5-FU alone was pocr,while in the group of 5- FU with LTA,not only the inhibition of tumor growth was more significant,but also the general status of mice was well improved, with increased count of blood leucocyte, SI, TI, level of T lymphocyte proliferation and IFN-γ in secretion of spleen cells. A lot of inflammatory cells could be observed under optic microscopy in LTA treatment group. Conclusion LTA of Bifidobacterium in combination with 5-FU could enhance anti-tumor effect and reverse the immunosuppression caused by chemotherapy with 5-FU. The present study suggested that LTA of Bifidobacterium is effective in enhancing the efficacy and reducing the toxicity of chemotherapy.