光合细菌2c菌株益生特性的实验研究

目的研究沼泽红假单胞菌2c菌株益生特性。方法利用耐酸、耐胆盐、体外黏附、平板抑菌试验及大鼠摄入2c菌株后在其体内的存活和持续时间实验研究其益生特性。结果pH2.5条件下处理30min及0.5%或0.9%牛胆盐处理对2c菌株活菌数无显著影响(P0.05);2c菌株体外对4株临床致病菌无抑制作用,体外黏附性能一般。2c菌株在大鼠体内存活和持续时间与摄入剂量有关,停止摄入3d后便不能富集出。结论2c菌株具有较好益生特性。

Investigation on probiotic properties of Photosynthetic bacteria strain 2c

Objective To investigate on the probiotic properties of Photosynthetic bacteria strain 2c. Method The resistance ability of strain 2c to acid and ox bile salt along with the property of adhesion to HT-29 cell and inhibiting pathogenic bacteria in vitro were assessed using routine methods. The survival and persistance time in vivo after intake by rat were also studied with bacteria culture.Result 30 min treatment with pH 2.5 and 0.5% or 0.9% ox bile salt had no significant effect on the survival of strain 2c（P〉0.05）.The strain 2c had no inhibiting ability to 4 clinical common pathogenic bacteria. The adhesion ability of strain 2c to HT-29 cell was common. The survival and standing time of 2c in vivo after intaken by rat was relative to intake content.The strain 2c couldn＇t be enriched 3 days after intaken. Conclusion The strain 2c has preferable probiotic properties.