| 基于16S rRNA基因和细菌基因组间重复序列的DNA指纹图谱技术对肝硬化大鼠肝移植后肠道菌群多样性的研究 |
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| 引用本文： | 刘霞,刘社兰,解奕瑞,阮冰.基于16S rRNA基因和细菌基因组间重复序列的DNA指纹图谱技术对肝硬化大鼠肝移植后肠道菌群多样性的研究[J].中国微生态学杂志,2010,22(3):193-198. |
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| 作者姓名： | 刘霞 刘社兰 解奕瑞 阮冰 |
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| 作者单位： | 1. 浙江大学医学院附属第一医院,传染病诊治国家重点实验室,浙江,杭州,310003
2. 杭州市疾病预防控制中心,浙江,杭州,310021 |
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| 摘 要： | 目的应用PCR-DGGE和rep-PCR技术对肝硬化大鼠肝移植后肠道菌群多样性进行研究,探讨肠道菌群多样性的变化,并比较这两种方法在菌群分析中的作用。方法首先建立CCL4诱导肝硬化大鼠的肝移植模型,收取对照组、肝硬化成模时、肝移植后7 d和肝移植后30 d的大鼠粪便,提取细菌基因组DNA,采用PCR-DGGE和rep-PCRBOX-PCR,ERIC-PCR,ERIC2-PCR,(GTG)5-PCR,REP-PCR]进行DNA指纹图谱分析。结果PCR-DGGE可明确将正常大鼠、肝硬化大鼠、肝移植大鼠分为3个簇,并显示出肝硬化、肝移植大鼠肠道菌群多样性明显增多。rep-PCR技术也可将各组分开,其中ERIC-PCR、ERIC2-PCR及REP-PCR三者扩增条带各组差异有显著性,鉴别效果更好。结论应用基于16S rRNA基因和细菌基因组间重复序列的指纹图谱技术对肝硬化大鼠肝移植后肠道微生态的研究具有重要价值,可对临床肝硬化、肝移植患者肠道微生态制剂的使用起到指导作用。
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| 关 键 词： | 肝硬化 肝移植 PCR-DGGE rep-PCR 菌群多样性 |
Dynamics of intestinal bacterial diversity in liver transplant rat with hepatic cirrhosis by PCR-DGGE and rep-PCR |
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| LIU Xia,LIU She-lan,XIE Yi-rui,RUAN Bing.Dynamics of intestinal bacterial diversity in liver transplant rat with hepatic cirrhosis by PCR-DGGE and rep-PCR[J].Chinese Journal of Microecology,2010,22(3):193-198. |
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| Authors: | LIU Xia LIU She-lan XIE Yi-rui RUAN Bing |
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| Institution: | LIU Xia1,LIU She-lan2,XIE Yi-rui1,RUAN Bing1 (1.State Key Laboratory for Diagnosis , Treatment of Infectious Diseases,the First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China,2.Center for Disease Control , Prevention of Hangzhou,Hangzhou 310021,China) |
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| Abstract: | Objective To explore the diversity of intestinal microbiota in liver transplantation model with hepatic cirrhosis by PCR-DGGE and rep-PCR and compare the two methods with each other.Method Hepatic cirrhosis model was established in rats by subcutaneous injection of 40% carbon tetrachloride and liver transplantation model was made with two-cuff technique.Then the feces in the following groups were collected: control group(A),hepatic cirrhosis model group(B),7 days after liver transplantation(C) and 30 days a... |
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| Keywords: | PCR-DGGE rep-PCR Cirrhosis Liver transplantation PCR-DGGE rep-PCR Bacterial diversity |
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