| 核心岩藻糖基转移酶Fut8基因敲除小鼠肠道菌群特征 |
| |
| 引用本文： | 李明,白亚强,何春阳,鄢秋龙,房慧,李志,袁杰力,李文哲.核心岩藻糖基转移酶Fut8基因敲除小鼠肠道菌群特征[J].中国微生态学杂志,2017(4). |
| |
| 作者姓名： | 李明 白亚强 何春阳 鄢秋龙 房慧 李志 袁杰力 李文哲 |
| |
| 作者单位： | 大连医科大学,大连医科大学,大连医科大学,大连医科大学,大连医科大学,大连市中心医院,大连医科大学,大连医科大学 |
| |
| 摘 要： | 目的探究Fut8基因敲除对ICR小鼠肠道菌群结构的影响。方法分别在Fut8~(+/+)和Fut8~(-/-)小鼠出生后不同时间点检测体重,采集粪便样本,采用PCR-变性梯度凝胶电泳(PCR-DGGE)的方法检测小鼠粪便中菌群的组成;利用高效液相色谱法(HPLC)测定Fut8~(+/+)和Fut8~(-/-)小鼠Fut8酶活。结果 Fut8~(-/-)小鼠与Fut8~(+/+)小鼠相比生长缓慢(P0.05);完全丧失核心岩藻糖基化活性;肠道菌群结构不同,其中拟杆菌门微生物存在显著差异。结论核心岩藻糖基转移酶Fut8基因敲除对小鼠肠道菌群结构有显著影响。
|
| 关 键 词： | 核心岩藻糖基转移酶 Fut8基因 基因敲除 肠道菌群 核心岩藻糖 |
Intestinal microbial structure of
core fucosyltransferase Fut8 gene knockout mice |
| |
| Abstract: | Abstract: Objective To determine the effect of Fut8 gene knockout on the structure of intestinal microbiota in ICR mice. Methods The body weights were measured at different time points after Fut8+/+ and Fut8-/ - mice were born. Fecal samples were collected at the same time to detect the intestinal microbiota by using PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The enzyme activity of Fut8 was assayed by using High Performance Liquid Chromatography (HPLC). Results The Fut8-/- mice grew slowly compared with Fut8+/+ mice (P <0.05), and the fucosyltransferase activity was abolished. Compared with the controls, the intestinal microbiota of Fut8-/- mice showed a different pattern, among which the bacteria that belong to the phylum of Bacteroidetes changed significantly. Conclusion Core fucosyltransferase (Fut8) gene knockout had a significant effect on the structure of intestinal microbiota in mice. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国微生态学杂志》浏览原始摘要信息 |
| 点击此处可从《中国微生态学杂志》下载免费的PDF全文 |
|
