黄连素对变异链球菌抑制作用的体外研究

目的分析黄连素对体外变异链球菌生长、产酸、粘附的影响,探讨其防龋作用。方法采用微量肉汤稀释法进行最小抑菌浓度(MIC)的测定;然后通过试管粘附法测定不同浓度药液对变异链球菌粘附作用的影响;最后计算不同浓度药液作用24h后pH值的变化。结果黄连素对变异链球菌的MIC为1.25mg/mL,MBC为5.00mg/mL。实验组对变异链球菌的粘附及产酸的抑制作用随着药物浓度的增加而增强,与阴性对照组相比差异有统计学意义(P0.05)。结论黄连素可抑制体外变异链球菌的生长、产酸及粘附。

The inhibitory effect of berberine on Streptococcus mutans in vitro

Abstract: Objective To analyze the effect of berberine on the growth, acid production and adhesion of Streptococcus mutans in vitro, and explore its role in the prevention of dental caries. Methods The minimum inhibitory concentration (MIC) was determined by the broth dilution method. The effects of different concentrations of the liquid on the adhesion of Streptococcus mutans were determined by tube adhesion method. The changes of pH values after 24 hours of treatment with different concentrations of the liquid were calculated. Results The MIC of berberine to Streptococcus mutans was 1.25 mg/mL and the MBC was 5.00 mg/mL. The inhibitory effect on the adhesion of Streptococcus mutans and the inhibition on acid production in the observation group increased with the increase of drug concentration, which was significantly different from that in the negative control group (P<0.05). Conclusion Berberine can inhibit the growth, acid production and adhesion of Streptococcus mutans in vitro.