双歧杆菌脂磷壁酸和总DNA对小鼠免疫功能的影响

目的比较双歧杆菌2种组分即双歧杆菌脂磷壁酸（LTA）和总DNA的免疫调节作用。方法分别提取和制备双歧杆菌LTA和总DNA。采用淋巴细胞转化法和溶血空斑法分别研究它们对小鼠细胞和体液免疫的调节作用。结果与对照组比较，双歧杆菌和总DNA对T细胞和B细胞都有明显的刺激作用（P〈0．05或P〈0．01），但是LTA的作用更强（P〈0．01）。结论双歧杆菌细胞壁的LTA和细胞核的总DNA均具有免疫调节作用，但前者效能优于后者。

Effects of lipoteichoic acid and total DNA of Bifidobacteria on immunological function of mice

Objective To compare the immunological effects of two kinds of components of Bifidobacterium bifidum such as lipoteichoic acid(LTA) and total DNA. Method LTA and total DNA of Bifidobacterium bifidum were extracted and prepared with chemical methods. Lymphocyte transforming assay and Plaque forming assay were used to evaluate the cellular and humoral immunoloregulation of above anaerobic preparations. Result Compared with the control,both LTA and total DNA of Bifidobacterium bifidum could stimulate significantly T cells and B cells (P ＜0.05 or P ＜0.01 ) ,but LTA was better than total DNA(P ＜0.01 ). Conclusion Both LTA and total DNA of Bifidobecterium bifidum can increase the immunomodulating effect,but the efficacy of the former is better than the latter.