高表达人核糖核酸酶抑制因子的乳腺癌细胞株的建立及其鉴定

目的建立稳定高表达人核糖核酸酶抑制因子（hRI）的真核细胞系：乳腺癌细胞系,为进一步研究hRI抗肿瘤、抗氧化的作用机制奠定实验基础。方法将hRI通过逆转录病毒载体（pLNCX-hRI）经过病毒包装细胞（PA317）包装后的高滴度病毒上清,感染乳腺癌（MCF-7）细胞系,经G418筛选后,运用RT-PCR、Western blot等方法进行鉴定hRI在MCF-7中的高表达。结果hRI克隆到乳腺癌细胞（MCF-7）基因组,并随着基因组稳定高表达hRI。结论利用逆转录病毒载体感染真核细胞后获得高表达hRI的肿瘤细胞株,从而为进一步研究真核细胞内hRI的抗肿瘤作用机制提供条件。

Establishment and identification of breast cancer cell line with over-expressed human ribonuclease inhibitor

Objective To establish a stable breast cancer cell line with over expressed human ribonuclease inhibtor （hRI）, and give a base on studying the anti-tumor and anti-oxidative function of hRI. Methods An infection of hRI gene into human breast carcinoma cell line： MCF-7, by the retroviral packaging cell line PA317 which carrying the pLNCX-hRI ,then MCF-7/pLNCX-hRI cell line with a stably higher expression of hRI was selected by G418 ,and then detected the over expression of hRI by RT-PCR, Western blot, et al. Results The study showed that hRI gene integrated into the ge- nome of the MCF-7 cell,and also hRI over-expressed in the infected MCF-7 cell line with its own genome. Conclusion The results suggest that hRI integrated into the MCF-7 cell line successfully, and the establishment of MCF-7/hRI cell line provided the condition for the further study of the mechanism of anti-tumor function of hRI.