Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods |
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| Authors: | J Fiala DR Lloyd M Rychtera CA Kent M Al-Rubeai |
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| Institution: | (1) Centre for Bioprocess Engineering, School of Chemical Engineering, The University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK;(2) Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Technicka 5, 166 28 Prague 6, Czech Republic |
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| Abstract: | Four counting methods (two flow cytometry, one Coulter principle, one microscopic) are compared for measuring cell density and viability of batch-grown yeast. All gave adequate precision in measuring total cell density with no systematic difference between methods. However, the promise of flow cytometry as a rapid means of determining both quantity and quality of a cell population is shown. |
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| Keywords: | cell number Coulter principle flow cytometry viability yeast |
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