Comparison of two anti-T-2 toxin immunoglobulins by direct ELISA |
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Authors: | Douglas B Rivers F Reese Frazer III Daniel R Coleman |
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Institution: | (1) Southern Research Institute, P.O. Box 55305, 35255-5305 Birmingham, AL |
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Abstract: | Summary Monoclonal anti-T-2 IgGs produced from 12C12 and 15H6 hybridomas were compared by enzyme-linked immunosorbent assay. Binding activity was linear from 0.005 to 0.25 g protein/ml with 12C12 and from 0.005 to 0.09 g protein/ml with 15H6. The quantity of T-2 toxin (g protein/ml) required for one-half maximum binding activity of 15H6 (0.0875 g protein/ml) was approximately 68% that of 12C12 (g protein/ml). |
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