Capillary electrophoresis of caseino-peptides: influence of applied voltage and column temperature |
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Authors: | Queiroz Macedo Pascale Jovilet Jean-Claude Meunier |
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Institution: | (1) Department of Chemistry, University of Aveiro, 3810 Aveiro, Portugal;(2) INRA, Laboratoire de Chimie Biologique, Centre de Biotechnologies Agro-Industrielles, INA Paris-Grignon, France |
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Abstract: | The peptides formed upon action of purified cardoon rennet on the Ala189-Phe190-Leu191-Leu192-Tyr193 -casein fragment were separated by capillary electrophoresis in an uncoated fused silica capillary. There was a linear correlation between electrophoretic mobility and Z/M2/3 (Z, net charge; M, molecular mass) under all experimental conditions tested; under the optimal condicitions, 25 kV and 40 °C, the correlation coefficient was 0.994. The reported method is fast (migration times less than 7 min) and may be used to study the action of aspartic proteinases on the C-terminal domain of -casein and thus to help elucidate their effect on cheese quality. |
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Keywords: | aspartic proteinase cardosin casein CZE peptide charge peptide size |
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