Purification of plasmid DNA by an improved electrophoretic protocol |
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| Authors: | E Szegedi Gy Varadi L Otten |
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| Institution: | (1) Research Institute for Viticulture and Enology, 6000 Kecskemét, P.O. Box 25, Hungary;(2) Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, 67084 Strasbourg, France |
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| Abstract: | Plasmid DNA from Escherichia coli was isolated by electroelution carried out in an agarose gel that contains an incorporated dialysis membrane. As the relative mobility of circular plasmid DNA to linear chromosomal DNA increases when the agarose concentration is decreased, we were able to purify plasmids of up to 50 kbp in 0.3% agarose gel in Tris acetate buffer yielding 10-60  g DNA ml bacterial culture. |
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