Protection of bioreactor from microbial contamination by high dissolved oxygen tension |
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Authors: | Seigo Sato Toshio Yajima Sukekuni Mukataka Joji Takahashi |
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Institution: | (1) Institute of Applied Biochemistry, University of Tsukuba, 305 Tsukuba, Ibaraki, Japan;(2) Faculty of engineering, Kogakuin University, Nishishinjuku Shinjuku-ku, 163-91 Tokyo, Japan |
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Abstract: | Summary Repeated batch hydrolysis of casein using immobilized protease were carried out at various do tensions and pH. At any of the pH tested, microbial contamination could be satisfactorily suppressed by maintaining high do. In continuous reaction, contamination could be suppressed throughout 40 days under the do of 190 ppm, whereas contamination was observed after 24 hours with the do of 2.5 ppm. |
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