Partition of proteins in aqueous two-phase systems containing charged dextran or hydrophobic PEG |
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| Authors: | Xijun Chai Meifang Jin Chengzhang Jiang |
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| Institution: | (1) Department of Membrane Process, Dalian Institute of Chemical Physics Chinese Academy of Sciences, P. O. Box 110, 116023 Dalian, P. R. China |
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| Abstract: | Summary The electrochemical effect of a charged dextran derivative and the hydrophobic effect of hydrophobic chain PEG derivative on partitioning of six types of proteins in PEG/dextran aqueous two-phase systems were investigated- When 1. 6%(w/w)DEAE-dextran was present in the system,the partition coefficient decreased quickly with increasing pH value;when 0. 4% (w/w)PEG pentadecanoic acid ester was present in the system, the partition coefficient of protein with strong hydrophobicity was greatly increased. The experimental results show that the influence of hydrocarbon chain PEG derivative on partition coefficient is closely related to the hydrophobicity of proteins. |
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